Preparing the Cuvette and Placing the Sample in the Spectrophotometer
A cuvette is filled with sample and placed in the Spectronic 20 sample compartment. The 12 thumbnail images summarize the content of the video. Click an image to see the image gallery.
Rinse the cuvette several times with your sample solution. Wipe the outside of the cuvette with a laboratory tissue to remove fingerprints. Check for air bubbles in the sample. Insert the cuvette into the sample compartment. Be sure the alignment marks line up close. Close the sample compartment lid.
Use a clean cuvette that is free of chips and cracks (though a cuvette with a chip near the top could be used).
Fill the cuvette at least half full. If there are air bubbles in the sample, gently tap the cuvette to displace the bubbles. If the bubbles remain, thoroughly clean the cuvette, rinse it with sample solution, and refill it. Don't forget to re-wipe the outside of the cuvette with a laboratory tissue.
Fingerprints and small amounts of liquid on the outer surface of the cuvette may cause inaccurate and inconsistent readings. In addition, small amounts of liquid would be transferred to the spectrophotometer potentially damaging the spectrophotometer.
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