14.9 Conjugation, Color, and the Chemistry of Vision
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Radical Quenching of 1,1-Diphenyl-2-picrylhydrazyl: A Spectrometric Determination of Antioxidant BehaviorJohn M. Berger, Roshniben J. Rana, Hira Javeed, Iqra Javeed, and Sandi L. Schulien Describes a colorimetric assay using the persistent radical 1,1-diphenyl-2-picrylhydrazyl to measure the activity of common antioxidants. This exercise is particularly appropriate for a course geared for nutrition or food science majors. Berger, John M.; Rana, Roshniben J.; Javeed, Hira; Javeed, Iqra; Schulien, Sandi L. J. Chem. Educ.2008, 85, 408.
Food Science |
Free Radicals |
UV-Vis Spectroscopy |
Vitamins
Synthesis of Triarylmethane and Xanthene Dyes Using Electrophilic Aromatic Substitution ReactionsJames V. McCullagh and Kelly A. Daggett In this experiment, electrophilic aromatic substitution reactions are used to synthesize several triarylmethane and xanthene dyes (fluorescein, erythrosin B, thymolphthalein, and rhodamine B) using common equipment while avoiding often troublesome, hydroscopic Lewis acids. Subsequent UVvis analysis produce spectra that match commercially available dye samples. McCullagh, James V.; Daggett, Kelly A. J. Chem. Educ.2007, 84, 1799.
Acids / Bases |
Aromatic Compounds |
Dyes / Pigments |
Electrophilic Substitution |
Equilibrium |
Synthesis |
UV-Vis Spectroscopy
An Inexpensive Kinetic Study: The Reaction of FD&C Red #3 (Erythrosin B) with HypochloriteMaher M. Henary and Arlene A. Russell Students use a desktop visible spectrophotometer to quantitatively follow the rate of disappearance of FD&C Red #3 with hypochlorite. The first-order reaction in both dye and bleach yields simple data that students can easily process and graph using spreadsheet software to obtain the rate constant and the rate law. Henary, Maher M.; Russell, Arlene A. J. Chem. Educ.2007, 84, 480.
Spectrophotometer, Scanning UV-Visible (ChemPages Lab)John W. Moore, Jerrold J. Jacobsen, Joe L. March Spectrophotometer, Scanning UV/Visible: this is a resource in the collection "ChemPages Laboratory Resources". A scanning ultraviolet/visible (UV/Vis) spectrophotometer operates on the same principles as a Spectronic 20?. They both can be used for qualitative and quantitative analysis. The ChemPages Laboratory Resources are a set of web pages that include text, images, video, and self check questions. The topics included are those that are commonly encountered in the first-year chemistry laboratory. They have been put together for use as both a pre-laboratory preparation tool and an in-laboratory reference source.
UV-Vis Spectroscopy |
Laboratory Equipment / Apparatus
Spectrometer, Spectronic 20 (ChemPages Lab)John W. Moore, Jerrold J. Jacobsen, Joe L. March Spectrometer, Spectronic 20?: this is a resource in the collection "ChemPages Laboratory Resources". The Spectronic 20? is used to measure the absorbance (or transmittance) of solutions. A Spectronic 20? is capable of measuring % transmittance and absorbance over the range of 340 to 950 nm (the range 600 to 950 nm requires a special infrared filter and a different lamp). The ChemPages Laboratory Resources are a set of web pages that include text, images, video, and self check questions. The topics included are those that are commonly encountered in the first-year chemistry laboratory. They have been put together for use as both a pre-laboratory preparation tool and an in-laboratory reference source.
