| Journal Articles: 24 results |
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A-DNA and B-DNA: Comparing Their Historical X-ray Fiber Diffraction Images Amand A. Lucas
This paper provides a comparative explanation of the structural content of the diffraction diagrams of A-DNA and B-DNA that facilitated the discovery of the double-helical structure of DNA by Watson and Crick in 1953. This analysis is supported a method that simulates both A-DNA and B-DNA X-ray images optically.
Lucas, Amand A. J. Chem. Educ. 2008, 85, 737.
Biophysical Chemistry |
Conformational Analysis |
Crystals / Crystallography |
X-ray Crystallography |
Nucleic Acids / DNA / RNA
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Using Physical Models of Biomolecular Structures To Teach Concepts of Biochemical Structure and Structure Depiction in the Introductory Chemistry Laboratory Gordon A. Bain, John Yi, Mithra Beikmohamadi, Timothy M. Herman, and Michael A. Patrick
Custom-made physical models of alpha-helices and beta-sheets, the zinc finger moiety, beta-globin, and green fluorescent protein are used to introduce students in first-year chemistry to the primary, secondary, and tertiary structure of proteins.
Bain, Gordon A.; Yi, John; Beikmohamadi, Mithra; Herman, Timothy M.; Patrick, Michael A. J. Chem. Educ. 2006, 83, 1322.
Amino Acids |
Proteins / Peptides |
Molecular Modeling |
Molecular Properties / Structure |
Nucleic Acids / DNA / RNA
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A Unique Demonstration Model of DNA Jonathan P. L. Cox
Describes a physical demonstration model of DNA for the classroom. The model comprises two types of building blocks that can be put together rapidly to produce an abstract structure that portrays several of the gross architectural features of idealized B-DNA.
Cox, Jonathan P. L. J. Chem. Educ. 2006, 83, 1319.
Molecular Biology |
Molecular Properties / Structure |
Student-Centered Learning |
Nucleic Acids / DNA / RNA
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DNA Profiling of the D1S80 Locus: A Forensic Analysis for the Undergraduate Biochemistry Laboratory D. Dewaine Jackson, Chad S. Abbey, and Dylan Nugent
Describes a laboratory exercise in DNA profiling that can be used to demonstrate four fundamental procedures: isolation of genomic DNA from human cells, use of the polymerase chain reaction to amplify DNA, separation of amplified DNAs on agarose and polyacrylamide gels, and quantitative analysis of data (while comparing two different gel separation techniques).
Jackson, D. Dewaine; Abbey, Chad S.; Nugent, Dylan. J. Chem. Educ. 2006, 83, 774.
Biological Cells |
Biotechnology |
Electrophoresis |
Forensic Chemistry |
Molecular Biology |
Quantitative Analysis |
Nucleic Acids / DNA / RNA
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Glycosyltransferases A and B: Four Critical Amino Acids Determine Blood Type Natisha L. Rose, Monica M. Palcic, and Stephen V. Evans
Human A, B, and O blood type is determined by the presence or absence of distinct carbohydrate structures on red blood cells. In this review the chemistry of the blood group ABO system and the role of glycosyltransferase A, glycosyltransferase B, and the four amino acids critical to determining blood group status are discussed.
Rose, Natisha L.; Palcic, Monica M.; Evans, Stephen V. J. Chem. Educ. 2005, 82, 1846.
Carbohydrates |
Enzymes |
Kinetics |
Bioorganic Chemistry |
Crystals / Crystallography |
Molecular Biology |
X-ray Crystallography |
Amino Acids
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Organic Chemistry of the Cell: An Interdisciplinary Approach To Learning with a Focus on Reading, Analyzing, and Critiquing Primary Literature Craig A. Almeida and Louis J. Liotta
Describes a sophomore-level learning community entitled Organic Chemistry of the Cell comprised of two linked courses, Organic Chemistry I and Cell Biology, and an Integrative Seminar. The Integrative Seminar is grounded in the reading, critical analysis, and discussion of primary literature that ties together organic chemistry and cell biology.
Almeida, Craig A.; Liotta, Louis J. J. Chem. Educ. 2005, 82, 1794.
Biological Cells |
Bioorganic Chemistry |
Drugs / Pharmaceuticals |
Enzymes |
Molecular Biology |
Student-Centered Learning
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Testing for Ultraviolet Toxicity Using Fungi Marcelo Vital and Patricia Esperón
In this article, we describe a laboratory project quantifying the effects of UV radiation on a suspension of living cells. This laboratory experiment was designed for undergraduate students in biochemistry. The experiment was carried out employing different fungi strains, changing the dosage of radiation, the distance to the UV source, and the exposure period to the UV radiation. The quantity of viable spores was calculated and the doseresponse curves were drawn. Students were able to prove that 100% of mortality was achieved with the longest time of exposure.
Vital, Marcelo; Esperón, Patricia. J. Chem. Educ. 2005, 82, 926.
Nucleic Acids / DNA / RNA |
UV-Vis Spectroscopy |
Medicinal Chemistry |
Molecular Biology |
Photochemistry
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Testing for Genetically Modified Foods Using PCR Ann Taylor and Samin Sajan
The polymerase chain reaction (PCR) is a powerful technique used to detect and amplify a specific DNA sequence. In this experiment, DNA is isolated from commercially available corn meal and corn-muffin mixes, then a sequence unique to transgenic plants is amplified by PCR. The amplified sequence is identified by its size (192 base pairs) by agarose gel electrophoresis. This experiment could be used in either a biochemistry course or in an analytical chemistry course in a curriculum that integrates biochemistry throughout the course work.
Taylor, Ann; Sajan, Samin. J. Chem. Educ. 2005, 82, 597.
Agricultural Chemistry |
Constitutional Isomers |
Biotechnology |
Plant Chemistry |
Consumer Chemistry |
Nucleic Acids / DNA / RNA
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A 3D Model of Double-Helical DNA Showing Variable Chemical Details Susan G. Cady
A 3D double-helical DNA model, made by placing beads on a wire and stringing beads through holes in plastic canvas, is described. Suggestions are given to enhance the basic helical frame to show the shapes and sizes of the nitrogenous base rings, 3' and 5' chain termini, and base pair hydrogen bonding. Students can incorporate random or real gene sequence data into their models.
Cady, Susan G. J. Chem. Educ. 2005, 82, 79.
Biotechnology |
Molecular Properties / Structure |
Molecular Modeling |
Nucleic Acids / DNA / RNA
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Identification of Forensic Samples via Mitochondrial DNA in the Undergraduate Biochemistry Laboratory Julie T. Millard and André M. Pilon
Identification of forensic samples via mitochondrial DNA in a hypothetical kidnapping case.
Millard, Julie T.; Pilon, André M. J. Chem. Educ. 2003, 80, 444.
Quantitative Analysis |
Electrophoresis |
Separation Science |
Biotechnology |
Forensic Chemistry |
Applications of Chemistry |
Nucleic Acids / DNA / RNA
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Genetic Code Wordsearch Terry L. Helser
Wordsearch puzzle containing 30 words that describe the nucleotide sequences used to produce proteins.
Helser, Terry L. J. Chem. Educ. 2003, 80, 417.
Biotechnology |
Nomenclature / Units / Symbols |
Enrichment / Review Materials |
Nucleic Acids / DNA / RNA
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Visualizing Atoms, Molecules, and Surfaces by Scanning Probe Microscopy Kimberly Aumann, Karen J. C. Muyskens, and Kumar Sinniah
Examples of applications of scanning probe microscopy (SPM) in an undergraduate curriculum; includes investigating DNA, analysis of microchip memory arrays and circuitry, and visualizing filamentous actin.
Aumann, Kimberly; Muyskens, Karen J. C.; Sinniah, Kumar. J. Chem. Educ. 2003, 80, 187.
Atomic Properties / Structure |
Instrumental Methods |
Materials Science |
Nanotechnology |
Surface Science |
Undergraduate Research |
Nucleic Acids / DNA / RNA
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Genomics: The Science and Technology Behind the Human Genome Project (by Charles R. Cantor and Cassandra L. Smith) reviewed by Martin J. Serra
This book is an outgrowth of a series of lectures given by one of the former heads (CRC) of the Human Genome Initiative. The book is designed to reach a wide audience, from biologists with little chemical or physical science background through engineers, computer scientists, and physicists with little current exposure to the chemical or biological principles of genetics.
Serra, Martin J. J. Chem. Educ. 2000, 77, 33.
Biotechnology |
Nucleic Acids / DNA / RNA
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Liver and Onions: DNA Extraction from Animal and Plant Tissues Karen J. Nordell, Anne-Marie L. Jackelen, S. Michael Condren, George C. Lisensky, and Arthur B. Ellis*
This activity, which allows students to extract DNA from plant and animal cells, serves as a spectacular example of the complexity of biochemical structure and function and fits well with a discussion of nucleic acids, hydrogen bonding, genetic coding, and heredity. DNA extraction can also be used in conjunction with a discussion of polymers and their properties.
Nordell, Karen J.; Jackelen, Anne-Marie L.; Condren, S. Michael; Lisensky, George C.; Ellis, Arthur B. J. Chem. Educ. 1999, 76, 400A.
Hydrogen Bonding |
Molecular Properties / Structure |
Nucleic Acids / DNA / RNA
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Chromatin Isolation and DNA Sequence Analysis in Large Undergraduate Laboratory Sections Ann E. Hagerman
One exercise is a simple laboratory experiment in which chromatin is isolated from chicken liver and is resolved into histone proteins and DNA by ion-exchange chromatography. The other is a series of computer simulations that introduce DNA sequencing, mapping, and sequence analysis to the students.
Hagerman, Ann E. J. Chem. Educ. 1999, 76, 1426.
Biotechnology |
Proteins / Peptides |
Nucleic Acids / DNA / RNA
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Research in Undergraduate Instruction: A Biotech Lab Project for Recombinant DNA Protein Expression in Bacteria Mark Brockman, Alfred B. Ordman, and A. Malcolm Campbell
In the sophomore-level Molecular Biology and Biotechnology course at Beloit College, students learn basic methods in molecular biology in the context of pursuing a semester-long original research project on how DNA sequence affects expression levels of proteins.
Brockman, Mark; Ordman, Alfred B.; Campbell, A. Malcom. J. Chem. Educ. 1996, 73, 542.
Molecular Biology |
Proteins / Peptides
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An Attack on the AIDS Virus: Inhibition of the HIV-1 Protease. New Drug Development Based on the Structure and Activity of the Protease and its Role in the Replication and Maturation of the Virus Volker, Eugene J.
Article shows how research into the biochemistry of HIV may lead to a method for controlling its growth and maturation. This research illustrates some basic enzyme chemistry and holds student appeal due to the tie into current affairs.
Volker, Eugene J. J. Chem. Educ. 1993, 70, 3.
Bioorganic Chemistry |
Proteins / Peptides |
Medicinal Chemistry |
Molecular Biology
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Are high school students ready for recombinant DNA?: The UOP experience Minch, Michael J.
What follows is a description of a three-week summer course on recombinant DNA offered to talented high school students between their junior and senior years.
Minch, Michael J. J. Chem. Educ. 1989, 66, 64.
Molecular Biology
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Nucleic acid structure and synthesis (Parish, J.H.) Parker, Keith K.
A review of a software program designed to introduce current understanding of DNA and RNA structure and the dynamic processes of replication and transcription.
Parker, Keith K. J. Chem. Educ. 1988, 65, A298.
Molecular Biology
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The biochemistry of brewing Bering, Charles L.
There are few topics that hold the attention of students as much as the one presented in this paper.
Bering, Charles L. J. Chem. Educ. 1988, 65, 519.
Biological Cells |
Carbohydrates |
Applications of Chemistry |
Alcohols |
Metabolism |
Enzymes |
Biotechnology |
Molecular Biology |
Consumer Chemistry
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Biopolymer models of nucleic acids Barrett, Edward J.
Space-filling models used to represent amino acids and the structure of DNA and RNA.
Barrett, Edward J. J. Chem. Educ. 1979, 56, 168.
Molecular Biology |
Molecular Modeling |
Molecular Properties / Structure
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Pheromones and chemical communication in the laboratory Alger, Donald B.
The topic of chemical communication is of great interest to biology students who may be taking courses in chemistry.
Alger, Donald B. J. Chem. Educ. 1976, 53, 210.
Hormones |
Bioorganic Chemistry |
Molecular Biology |
Molecular Recognition
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The Molecular Basis of Evolution (Anfinsen, Christian B.) Edsall, John T.
Edsall, John T. J. Chem. Educ. 1960, 37, 107.
Molecular Biology
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Isomers of octahedral complexes with nonbranching ligands Trimble, Russell F., Jr.
The purpose of this paper is to present a tabulation of the various ways in which ligands can be distributed about an octahedral frame.
Trimble, Russell F., Jr. J. Chem. Educ. 1954, 31, 176.
Coordination Compounds |
Molecular Biology |
Diastereomers
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