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Nature of Proteins Effect of pH on solubility, denaturing proteins, hydrolysis in strong base, binding to coomassie blue, dying wool and cotton, gel filtration column, reaction of ninhydrin with amino acids and precipitating protein with ammonium sulfate are demonstrated.
Proteins / Peptides
Protein Reactions Curdling milk, decomposition of hydrogen peroxide by catalase, glucose oxidase activity, hydrolyzing of sucrose with invertase, and using an enzyme to halt the Briggs-Rauscher reaction are demonstrated. Inhibiting the enzyme HIV-1 protease may slow the spread of AIDS.
Proteins / Peptides
Denaturation of Protein by Strong AcidJohn W. Moore, Jerrold J. Jacobsen, Gary Trammell, Kristin Johnson "Strong acid denatures egg white proteins.This video contains voiceover:""The tertiary structure of egg white proteins is held together by hydrogen bonding and hydrophobic interactions. Adding strong acid to the protein disrupts the intermolecular forces, and the tertiary structure is lost. The loss in protein structure is called denaturation. The proteins precipitate, forming a white solid."""
Soup or Salad? Investigating the Action of Enzymes in Fruit on GelatinErica Jacobsen Some fruits contain proteases, a group of enzymes that catalyze the cleavage of peptide linkages in proteins. This can have an undesired effect in a gelatin salad containing fruit, because proteases in the fruit can cleave the proteins that make up the structure of gelatin so that it will not set. Jacobsen, Erica. J. Chem. Educ.1999, 76, 624A.
Enzymes |
Proteins / Peptides
Analysis of Iron in Ferritin, the Iron-Storage Protein: A General Chemistry ExperimentMaureen J. Donlin, Regina F. Frey, Christopher Putnam, Jody Proctor, and James K. Bashkin The chemical properties of the iron-storage protein ferritin will be examined in this experiment by quantitating the average amount of iron in a ferritin sample, and observing the kinetics of iron release from the protein core under reducing conditions. Students learn and apply the principles of absorption spectroscopy, redox reactions, and metal chelation to study ferritin. Maureen J. Donlin, Regina F. Frey, Christopher Putnam, Jody Proctor, and James K. Bashkin. J. Chem. Educ.1998, 75, 437.
Oxidation / Reduction |
UV-Vis Spectroscopy |
Proteins / Peptides
Protein denaturation: A physical chemistry project labPickering, Miles; Crabtree, Robert H. This experiment links physical chemistry with biology and can be done with in advanced freshman course. Pickering, Miles; Crabtree, Robert H. J. Chem. Educ.1981, 58, 513.
Proteins / Peptides |
Biophysical Chemistry |
Molecular Properties / Structure
Other Resources: First 3 results
Umami and ProteinsRobert Hetue A section of ChemPrime, the Chemical Educations Digital Library's free General Chemistry textbook.
Proteins / Peptides
ChemPaths 104 M Jan 31John W. Moore Today in Chem 104:
* Lecture: Biochemistry: Proteins
* Reading:
Kotz: Chemistry of Life (pp. 496-502);
Moore: Ch 3, Sec 11; Ch 12, Sec 7
* Biomolecules Tutorials
o Proteins 1 (including debriefing)
o Proteins 2 (including debriefing)
o Quiz due W Feb. 2, 11:55 PM)
* Homework #3 due by 11:55pm F Feb 4.
Proteins / Peptides
Polypeptide ChainsEd Vitz, John W. Moore A section of ChemPrime, the Chemical Educations Digital Library's free General Chemistry textbook.
