TIGER

Journal Articles: 8 results
A-DNA and B-DNA: Comparing Their Historical X-ray Fiber Diffraction Images  Amand A. Lucas
This paper provides a comparative explanation of the structural content of the diffraction diagrams of A-DNA and B-DNA that facilitated the discovery of the double-helical structure of DNA by Watson and Crick in 1953. This analysis is supported a method that simulates both A-DNA and B-DNA X-ray images optically.
Lucas, Amand A. J. Chem. Educ. 2008, 85, 737.
Biophysical Chemistry |
Conformational Analysis |
Crystals / Crystallography |
X-ray Crystallography |
Nucleic Acids / DNA / RNA
DNA Profiling of the D1S80 Locus: A Forensic Analysis for the Undergraduate Biochemistry Laboratory  D. Dewaine Jackson, Chad S. Abbey, and Dylan Nugent
Describes a laboratory exercise in DNA profiling that can be used to demonstrate four fundamental procedures: isolation of genomic DNA from human cells, use of the polymerase chain reaction to amplify DNA, separation of amplified DNAs on agarose and polyacrylamide gels, and quantitative analysis of data (while comparing two different gel separation techniques).
Jackson, D. Dewaine; Abbey, Chad S.; Nugent, Dylan. J. Chem. Educ. 2006, 83, 774.
Biological Cells |
Biotechnology |
Electrophoresis |
Forensic Chemistry |
Molecular Biology |
Quantitative Analysis |
Nucleic Acids / DNA / RNA
Glycosyltransferases A and B: Four Critical Amino Acids Determine Blood Type  Natisha L. Rose, Monica M. Palcic, and Stephen V. Evans
Human A, B, and O blood type is determined by the presence or absence of distinct carbohydrate structures on red blood cells. In this review the chemistry of the blood group ABO system and the role of glycosyltransferase A, glycosyltransferase B, and the four amino acids critical to determining blood group status are discussed.
Rose, Natisha L.; Palcic, Monica M.; Evans, Stephen V. J. Chem. Educ. 2005, 82, 1846.
Carbohydrates |
Enzymes |
Kinetics |
Bioorganic Chemistry |
Crystals / Crystallography |
Molecular Biology |
X-ray Crystallography |
Amino Acids
Organic Chemistry of the Cell: An Interdisciplinary Approach To Learning with a Focus on Reading, Analyzing, and Critiquing Primary Literature  Craig A. Almeida and Louis J. Liotta
Describes a sophomore-level learning community entitled Organic Chemistry of the Cell comprised of two linked courses, Organic Chemistry I and Cell Biology, and an Integrative Seminar. The Integrative Seminar is grounded in the reading, critical analysis, and discussion of primary literature that ties together organic chemistry and cell biology.
Almeida, Craig A.; Liotta, Louis J. J. Chem. Educ. 2005, 82, 1794.
Biological Cells |
Bioorganic Chemistry |
Drugs / Pharmaceuticals |
Enzymes |
Molecular Biology |
Student-Centered Learning
Testing for Genetically Modified Foods Using PCR  Ann Taylor and Samin Sajan
The polymerase chain reaction (PCR) is a powerful technique used to detect and amplify a specific DNA sequence. In this experiment, DNA is isolated from commercially available corn meal and corn-muffin mixes, then a sequence unique to transgenic plants is amplified by PCR. The amplified sequence is identified by its size (192 base pairs) by agarose gel electrophoresis. This experiment could be used in either a biochemistry course or in an analytical chemistry course in a curriculum that integrates biochemistry throughout the course work.
Taylor, Ann; Sajan, Samin. J. Chem. Educ. 2005, 82, 597.
Agricultural Chemistry |
Constitutional Isomers |
Biotechnology |
Plant Chemistry |
Consumer Chemistry |
Nucleic Acids / DNA / RNA
Identification of Forensic Samples via Mitochondrial DNA in the Undergraduate Biochemistry Laboratory  Julie T. Millard and André M. Pilon
Identification of forensic samples via mitochondrial DNA in a hypothetical kidnapping case.
Millard, Julie T.; Pilon, André M. J. Chem. Educ. 2003, 80, 444.
Quantitative Analysis |
Electrophoresis |
Separation Science |
Biotechnology |
Forensic Chemistry |
Applications of Chemistry |
Nucleic Acids / DNA / RNA
Genomics: The Science and Technology Behind the Human Genome Project (by Charles R. Cantor and Cassandra L. Smith)  reviewed by Martin J. Serra
This book is an outgrowth of a series of lectures given by one of the former heads (CRC) of the Human Genome Initiative. The book is designed to reach a wide audience, from biologists with little chemical or physical science background through engineers, computer scientists, and physicists with little current exposure to the chemical or biological principles of genetics.
Serra, Martin J. J. Chem. Educ. 2000, 77, 33.
Biotechnology |
Nucleic Acids / DNA / RNA
Chromatin Isolation and DNA Sequence Analysis in Large Undergraduate Laboratory Sections  Ann E. Hagerman
One exercise is a simple laboratory experiment in which chromatin is isolated from chicken liver and is resolved into histone proteins and DNA by ion-exchange chromatography. The other is a series of computer simulations that introduce DNA sequencing, mapping, and sequence analysis to the students.
Hagerman, Ann E. J. Chem. Educ. 1999, 76, 1426.
Biotechnology |
Proteins / Peptides |
Nucleic Acids / DNA / RNA