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Journal Articles: 25 results
OMLeT—An Alternative Approach to Learning Metabolism: Glycolysis and the TCA Cycle as an Example  Charles M. Stevens, Dylan M. Silver, Brad Behm, Raymond J. Turner, and Michael G. Surette
Using PHP Hypertext Preprocessor scripting, the dynamic OMLeT (Online Metabolism Learning Tool) Web site is geared towards different learning styles and allows the student to process metabolic pathways (glycolysis and TCA cycle) via a user-defined approach.
Stevens, Charles M.; Silver, Dylan M.; Behm, Brad; Turner, Raymond J.; Surette, Michael G. J. Chem. Educ. 2007, 84, 2024.
Bioenergetics |
Enzymes |
Learning Theories |
Metabolism |
Proteins / Peptides
Some Like It Cold: A Computer-Based Laboratory Introduction to Sequence and Tertiary Structure Comparison of Cold-Adapted Lactate Dehydrogenases Using Bioinformatics Tools  M. Sue Lowery and Leigh A. Plesniak
Students download sequences and structures from appropriate databases, create sequence alignments, and carry out molecular modeling exercises, and then form hypotheses about the mechanism of biochemical adaptation for function and stability. This laboratory is appropriate for biochemistry and molecular biology laboratory courses, special topics, and advanced biochemistry lecture courses, and can be adapted for honors high school programs.
Lowery, M. Sue; Plesniak, Leigh A. J. Chem. Educ. 2003, 80, 1300.
Enzymes |
Molecular Modeling |
Proteins / Peptides |
Molecular Properties / Structure
Collaboration between Chemistry and Biology to Introduce Spectroscopy, Electrophoresis, and Molecular Biology as Tools for Biochemistry  Vicky L. H. Bevilacqua, Jennifer L. Powers, Connie Tran, Swapan S. Jain, Reem Chabayta, Dale L. Vogelien, Ralph J. Rascati, Michelle Hall, and Kathleen Diehl
Program that integrates a variety of instrumental techniques across the biological and chemistry curricula, including biochemistry, plant physiology, genetics, and forensics.
Bevilacqua, Vicky L. H.; Powers, Jennifer L.; Vogelien, Dale L.; Rascati, Ralph J.; Hall, Michelle; Diehl, Kathleen; Tran, Connie; Jain, Swapan S.; Chabayta, Reem . J. Chem. Educ. 2002, 79, 1311.
Biotechnology |
Enzymes |
Forensic Chemistry |
Hormones |
Instrumental Methods |
Kinetics |
Plant Chemistry |
Proteins / Peptides |
UV-Vis Spectroscopy
Factors Affecting Reaction Kinetics of Glucose Oxidase  Kristin A. Johnson
Demonstration based on a biochemical kinetics experiment in which the rate of reaction varies with the enzyme concentration, substrate concentration, substrate used in the reaction, and temperature.
Johnson, Kristin A. J. Chem. Educ. 2002, 79, 74.
Enzymes |
Kinetics |
Proteins / Peptides |
Carbohydrates |
Catalysis |
Rate Law
A Simple Method for Demonstrating Enzyme Kinetics Using Catalase from Beef Liver Extract  Kristin A. Johnson
A simple visual method of demonstrating enzyme kinetics using beef liver catalase. Filter paper is saturated with beef liver extract and placed into a solution of hydrogen peroxide. The catalase in the extract decomposes the hydrogen peroxide to water and oxygen. Oxygen forms on the filter paper, and the filter paper rises to the top of the beaker. Catalase activity is measured by timing the rise of the enzyme-soaked filter paper to the top of beakers containing different concentrations of hydrogen peroxide.
Johnson, A. Kristin. J. Chem. Educ. 2000, 77, 1451.
Enzymes |
Kinetics |
Proteins / Peptides |
Reactions
Homogeneous Immunoassays: Historical Perspective and Future Promise  Edwin F. Ullman
The founding and growth of Syva Company is examined in the context of its leadership role in the development of homogeneous immunoassays. The simple mix and read protocols of these methods offer advantages in routine analytical and clinical applications.
Ullman, Edwin F. J. Chem. Educ. 1999, 76, 781.
Biotechnology |
Enzymes |
Atomic Properties / Structure |
Free Radicals |
Photochemistry |
Medicinal Chemistry |
Proteins / Peptides
The Well-Read Biochemist  Gale Rhodes
This article describes how I use a collection of poems, essays, and fiction to inspire my biochemistry students to make connections between the arcane world of cellular chemistry and the wider world of literature and the humanities.
Rhodes, Gale. J. Chem. Educ. 1996, 73, 732.
Enzymes |
Kinetics |
Metabolism |
Proteins / Peptides |
Membranes
Biochemical Data on the Web  Barmettler, Peter
186. Searching for information about enzymes, receptors, and other biochemical data is a painstaking task for chemists even in well-equipped libraries. The World Wide Web (WWW), also called the information superhighway, offers now an alternative approach, which is especially advantageous for homology modeling.
Barmettler, Peter J. Chem. Educ. 1996, 73, 520.
Enzymes |
Drugs / Pharmaceuticals |
Proteins / Peptides |
Receptors
A Problem-Based Learning Design for Teaching Biochemistry  Richard F. Dods
This article describes the design of a biochemistry course that uses problem-based learning. Examples of some of the problems incorporated into the course are described in detail.
Dods, Richard F. J. Chem. Educ. 1996, 73, 225.
Proteins / Peptides |
Enzymes |
Learning Theories
Breaking Bonds versus Chopping Heads: The Enzyme as Butcher  Todd P. Silverstein
Analogy to help biochemistry students understand concepts of Michaelis-Menten kinetics; active site/binding site; activation energy; substrate saturation; cooperativity; allosteric effects; and inhibitors.
Silverstein, Todd P. J. Chem. Educ. 1995, 72, 645.
Catalysis |
Enzymes |
Kinetics |
Proteins / Peptides |
Mechanisms of Reactions |
Reactions |
Rate Law
An Integrated Approach to the Undergraduate Biochemistry Laboratory   James G. Harman, John A. Anderson, Richard A. Nakashima, and Robert W. Shaw
Plan to improve the undergraduate biochemistry laboratory curriculum, including list and brief description of tutorials and experiments.
Harman, James G.; Anderson, John A.; Nakashima, Richard A.; Shaw, Robert W. J. Chem. Educ. 1995, 72, 641.
Enzymes |
Proteins / Peptides
Nucleophilic and Enzymic Catalysis of p-Nitrophenylacetate Hydrolysis  Head, Michael B.; Mistry, Kalpna S.; Ridings, Bernard J.; Smith, Christopher A.; Parker, Mark J.
Experimental procedure for determining the relative effectiveness of several amino acids and enzymes in catalyzing the hydrolysis of p-nitrophenylacetate; sample data and analysis included.
Head, Michael B.; Mistry, Kalpna S.; Ridings, Bernard J.; Smith, Christopher A.; Parker, Mark J. J. Chem. Educ. 1995, 72, 184.
Amino Acids |
Enzymes |
Proteins / Peptides |
Catalysis
Effects of "crowding" in protein solutions  Ralston, G. B.
The effects of macromolecular nonideality and crowding on chemical equilibria, association reactions, and enzyme kinetics.
Ralston, G. B. J. Chem. Educ. 1990, 67, 857.
Proteins / Peptides |
Equilibrium |
Reactions |
Enzymes |
Kinetics
An enzyme immunoassay for human transferrin  Russo, Salvatore F.; Dahlberg, Judy Utter
The field of immunology provides a powerful method for detecting specific noncatalytic proteins.
Russo, Salvatore F.; Dahlberg, Judy Utter J. Chem. Educ. 1990, 67, 175.
Enzymes |
Medicinal Chemistry |
Proteins / Peptides
Azosulfonamides: Preparation and binding to carbonic anhydrase: A bioorganic chemistry experiment  Manalang, Mary G.; Bundy, Hallie F.
Preparation of azosulfonamide, visible absorption analysis of free and enzyme-bond azosulfonamide, and titration of BCA with azosulfonamide.
Manalang, Mary G.; Bundy, Hallie F. J. Chem. Educ. 1989, 66, 609.
Bioorganic Chemistry |
Synthesis |
Proteins / Peptides |
Titration / Volumetric Analysis |
Enzymes
A convenient synthesis of aspartame  Lindeberg, Gunnar
The one tube, enzymatic synthesis of the dipeptide sweetener aspartame.
Lindeberg, Gunnar J. Chem. Educ. 1987, 64, 1062.
Synthesis |
Enzymes |
Proteins / Peptides
Use of the "cubic snake" as a molecular model  Gilon, Chaim
Using a "cubic snake" to model macromolecules.
Gilon, Chaim J. Chem. Educ. 1985, 62, 1074.
Molecular Modeling |
Molecular Properties / Structure |
Proteins / Peptides |
Enzymes
The catalytic function of enzymes  Splittgerber, Allan G.
Review of the structure, function, and factors that influence the action of enzymes.
Splittgerber, Allan G. J. Chem. Educ. 1985, 62, 1008.
Catalysis |
Enzymes |
Mechanisms of Reactions |
Proteins / Peptides |
Molecular Properties / Structure
Zinc enzymes  Bertini, I.; Luchinat, C.; Monnanni, R.
The role played by catalytic and noncatalytic zinc in biochemical systems. From the "State of the Art Symposium: Bioinorganic Chemistry", held at the ACS meeting, Miami, 1985.
Bertini, I.; Luchinat, C.; Monnanni, R. J. Chem. Educ. 1985, 62, 924.
Enzymes |
Bioinorganic Chemistry |
Lewis Acids / Bases |
Proteins / Peptides
Methemoglobinemia: An illness caused by the ferric state  Senozan, N. M.
Hemoglobin's ability to carry oxygen depends on the iron being in the +2 state; methemoglobinemia involves the oxidation of hemoglobin iron to the +3 state.
Senozan, N. M. J. Chem. Educ. 1985, 62, 181.
Proteins / Peptides |
Enzymes |
Medicinal Chemistry |
Oxidation / Reduction |
Oxidation State
Isoenzymes  Daugherty, N. A.
The separation, identification, and measurement of isoenzymes is an appropriate topic for a special lecture in general chemistry.
Daugherty, N. A. J. Chem. Educ. 1979, 56, 442.
Enzymes |
Proteins / Peptides |
pH |
Electrophoresis |
Separation Science |
Electrochemistry |
Applications of Chemistry
Bromolain. Experiments illustrating proteolytic enzyme action  Reigh, Darryel L.
The following set of exercises provides a simple visual method of demonstrations some of the characteristics of enzymes in general, such as heat and pH lability and inhibition, as well as some specific properties of bromelain intide hydrolysis. These experiments can be used with freshman in introductory courses and juniors in biochemistry.
Reigh, Darryel L. J. Chem. Educ. 1976, 53, 386.
Enzymes |
pH |
Food Science |
Natural Products |
Proteins / Peptides
Simple method for demonstrating an enzymatic reaction  Tang, Chung-Shih
Uses taste sensations of papaya seeds under varying conditions to demonstrate an enzymatic reaction.
Tang, Chung-Shih J. Chem. Educ. 1970, 47, 692.
Enzymes |
Proteins / Peptides |
Food Science |
Reactions |
Consumer Chemistry |
Applications of Chemistry
Hydrogen sulfide under any other name still smells. A poisonous story  Brasted, Robert C.
The chemistry of hydrogen sulfide affords an excellent opportunity to integrate descriptive inorganic and coordination chemistry with biochemistry.
Brasted, Robert C. J. Chem. Educ. 1970, 47, 574.
Descriptive Chemistry |
Molecular Properties / Structure |
Coordination Compounds |
Enzymes |
Proteins / Peptides
Disposable macromolecular model "kits"  Nicholson, Isadore
A brief note suggesting the use of colored pipe cleaners for the construction of three dimensional models of polymers, particularly enzymes and other proteins.
Nicholson, Isadore J. Chem. Educ. 1969, 46, 671.
Molecular Modeling |
Enzymes |
Proteins / Peptides