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Journal Articles: 27 results
Kinetic Analysis of Amylase Using Quantitative Benedict's and Iodine Starch Reagents  Beverly Cochran, Deborah Lunday, and Frank Miskevich
This laboratory emphasizes that enzymes mediate the conversion of a substrate into a product and that either the concentration of product or reactant may be used to follow the course of a reaction. It does so by using an inexpensive scanner and open-source image analysis software to quantify amylase activity through the breakdown of starch and the appearance of glucose.
Cochran, Beverly; Lunday, Deborah; Miskevich, Frank. J. Chem. Educ. 2008, 85, 401.
Biosynthesis |
Carbohydrates |
Catalysis |
Enzymes |
Food Science |
Nutrition |
Quantitative Analysis |
UV-Vis Spectroscopy
Incorporating Biological Mass Spectrometry Into Undergraduate Teaching Labs, Part 1: Identifying Proteins Based on Molecular Mass  Isaac J. Arnquist and Douglas J. Beussman
This article presents the first in a series of experiments designed to introduce students to the analysis of biological molecules using mass spectrometry. In this experiment, students analyze and identify a medium-sized protein using electrospray ionization.
Arnquist, Isaac J.; Beussman, Douglas J. J. Chem. Educ. 2007, 84, 1971.
Bioanalytical Chemistry |
Instrumental Methods |
Mass Spectrometry |
Proteins / Peptides |
Qualitative Analysis
Research Advances: Less Expensive and More Convenient Gaucher's Disease Treatment; Structural Loop Regions: Key to Multidrug-Resistance Transporters?; New Method Identifies Proteins in Old Artwork  Angela G. King
Molecular chaperones lead the way to new Gaucher's disease treatment. Researchers determine structure of the multidrug transporter EmrD. Whole egg proteins present in Renaissance art microsamples.
King, Angela G. J. Chem. Educ. 2006, 83, 1738.
Bioinorganic Chemistry |
Mass Spectrometry |
X-ray Crystallography |
Medicinal Chemistry |
Proteins / Peptides |
Bioorganic Chemistry
Mineral Analysis of Whole Grain Total Cereal  Paul Hooker
This article describes the quantitative analysis of the elements iron, zinc, and calcium in Whole Grain Total Cereal, a cereal product that claims to contain 100% of the daily value of several vitamins and minerals. This experiment can be implemented at several instructional levels including chemistry courses for science and nonscience majors, and in more advanced chemistry courses such as quantitative or instrumental analysis.
Hooker, Paul. J. Chem. Educ. 2005, 82, 1223.
Consumer Chemistry |
Food Science |
Nutrition |
Quantitative Analysis |
UV-Vis Spectroscopy |
Nonmajor Courses
Introduction to Biological Mass Spectrometry: Determining Identity and Species of Origin of Two Proteins  Curt T. Reimann, Axel Mie, Carina Nilsson, and Arieh Cohen
We describe a laboratory exercise that is designed to give advanced undergraduate students in analytical chemistry a meaningful introduction to biological mass spectrometry. We ask the students to solve a mystery: given two proteins, what are they, and from which animals do they originate? This exercise involves use of two mass spectrometers available in our institution: a fairly basic matrix-assisted laser desorption ionization time-of-flight (MALDITOF) unit and an electrospray ionization (ESI) ion-trap unit. Our exercise can be completed in two days (including analysis) and provides several interesting and educational surprises.
Reimann, Curt T.; Mie, Axel; Nilsson, Carina; Cohen, Arieh. J. Chem. Educ. 2005, 82, 1215.
Instrumental Methods |
Isotopes |
Mass Spectrometry |
Proteins / Peptides |
Bioanalytical Chemistry
Cytochrome c: A Biochemistry Laboratory Course  John B. Vincent and Stephen A. Woski
A unified biochemistry laboratory course has been developed. Rather than using miscellaneous laboratory experiments, the concept behind this course is to focus on a theme: the process of biochemical research. The laboratory deals entirely with one protein, cytochrome c, simulating the experience of a real-world research project. The laboratory is built around team-learning: groups of students isolate cytochrome c, characterize it by a variety of biochemical and bioinorganic methods, attempt to determine its function, and finally alter the protein to produce a modifier of oxidative nucleic acid damage.
Vincent, John B.; Woski, Stephen A. J. Chem. Educ. 2005, 82, 1211.
Bioinorganic Chemistry |
Proteins / Peptides |
Chromatography |
Electrophoresis |
Enzymes |
EPR / ESR Spectroscopy |
NMR Spectroscopy |
Nucleic Acids / DNA / RNA |
UV-Vis Spectroscopy
Determination of Minerals in Apples by ICP–AES  Mark Duxbury
A laboratory experiment is described that involves the elemental analysis of apples by inductively coupled plasmaatomic emission spectroscopy (IICPAES). The results of the experiment allow students to predict the cold-storage stability of apples.
Duxbury, Mark. J. Chem. Educ. 2003, 80, 1180.
Agricultural Chemistry |
Atomic Properties / Structure |
Consumer Chemistry |
Food Science |
Instrumental Methods |
Nutrition |
Atomic Spectroscopy
Identifying a Protein by MALDI–TOF Mass Spectrometry: An Experiment for the Undergraduate Laboratory  Anne E. Counterman, Matthew S. Thompson, and David E. Clemmer
Experiment that requires students to use mass spectral data (MALDI-TOF) to find the identity of an unknown protein sample by performing a database search on the Internet.
Counterman, Anne E.; Thompson, Matthew S.; Clemmer, David E. J. Chem. Educ. 2003, 80, 177.
Mass Spectrometry |
Proteins / Peptides |
Instrumental Methods |
Qualitative Analysis
Identification of Yeast V-ATPase Mutants by Western Blots Analysis of Whole Cell Lysates  Karlett Parra-Belky
Laboratory using three yeast strains to illustrate link between genetic engineering and biochemistry research.
Parra-Belky, Karlett. J. Chem. Educ. 2002, 79, 1348.
Biotechnology |
Electrophoresis |
Enzymes |
Proteins / Peptides |
Separation Science |
Applications of Chemistry
Testing the alpha-Amylase Inhibitor of the Common Bean: An Experiment on Selective Inhibition of Animal Enzymes by the Natural Defense Proteins of Plants  Moreno, Joaquin; Puig-Roque, Oscar; Nevado, Juan-Jose; Garcia-Ferris, Carlos
A procedure designed to test the selective inhibition of the bean alpha-amylase inhibitor against alpha-amylases from an animal (human saliva) and plant (germinated barley seeds).
Moreno, Joaquin; Puig-Roque, Oscar; Nevado, Juan-Jose; Garcia-Ferris, Carlos J. Chem. Educ. 1994, 71, 350.
Proteins / Peptides |
Enzymes
Nutrition: A Popular General Education Chemistry Course  Mathews, Frances
A course description for a popular nutrition course that includes elementary chemistry, biochemistry, and physiology. A course outline is included.
Mathews, Frances J. Chem. Educ. 1993, 70, 47.
Nutrition |
Bioenergetics |
Nonmajor Courses
Insulin: HPLC mapping of protease digestion products: A biochemistry laboratory experiment  Vestling, Martha M.
Students learn about about protein mapping through the activity written up in this article.
Vestling, Martha M. J. Chem. Educ. 1991, 68, 958.
HPLC |
Proteins / Peptides |
Enzymes
Analyzing DNA-protein interactions by gel electrophoresis  Revzin, Arnold
Quantitative and qualitative applications of the gel retardation assay in the study of DNA-protein interactions.
Revzin, Arnold J. Chem. Educ. 1990, 67, 749.
Proteins / Peptides |
Electrophoresis |
Quantitative Analysis |
Qualitative Analysis
Isolation of the active site of cytochrome c by reverse-phase high-performance liquid chromatography  Kenigsberg, Paul A.; Blanke, Steven R.; Hager, Lowell P.
To serve as an introduction to research, an advanced-level graduate laboratory course should be challenging, incorporate currently relevant techniques, offer more responsibility to students, and contain a minimum of technical pitfalls. The following experiment meets all the aforementioned criteria.
Kenigsberg, Paul A.; Blanke, Steven R.; Hager, Lowell P. J. Chem. Educ. 1990, 67, 177.
HPLC |
Proteins / Peptides
Preparation and characterization of myosin proteins  Caldwell, Elizabeth; Eftink, Maurice R.
Each student or team of lab partners is responsible for one of five projects involving the isolation and characterization of rabbit myosin proteins and its water-soluble subfragments.
Caldwell, Elizabeth; Eftink, Maurice R. J. Chem. Educ. 1985, 62, 900.
Synthesis |
Proteins / Peptides |
Electrophoresis |
Enzymes
Bomb calorimetry: The energy content of pizza  Stout, Roland P.; Nettleton, Faith E.; Price, Lynn M.
Determining the energy content and percent protein of pizza using bomb calorimetry.
Stout, Roland P.; Nettleton, Faith E.; Price, Lynn M. J. Chem. Educ. 1985, 62, 438.
Calorimetry / Thermochemistry |
Food Science |
Proteins / Peptides |
Titration / Volumetric Analysis
The chemical composition of the cell  Holum, John R.
A broad outline of the chemical substances that occur in most cells.
Holum, John R. J. Chem. Educ. 1984, 61, 877.
Biological Cells |
Lipids |
Carbohydrates |
Proteins / Peptides
Biochemistry for nutrition/dietetics students: Course content  Sirota, Lorraine Handler
Results regarding surveys of biochemistry resources and content for nutrition/dietetics students.
Sirota, Lorraine Handler J. Chem. Educ. 1984, 61, 430.
Nutrition |
Medicinal Chemistry |
Nonmajor Courses
The metabolism of carbohydrates  
Traces the metabolism of carbohydrates and the flow of materials among the various resource pools in living systems.
J. Chem. Educ. 1979, 56, 534.
Carbohydrates |
Metabolism |
Bioenergetics
Effects of ethanol on nutrition  Shorey, RoseAnn L.
The relationships between alcohol and obesity, malnutrition, vitamin and mineral absorption and utilization, and toxicity.
Shorey, RoseAnn L. J. Chem. Educ. 1979, 56, 532.
Alcohols |
Nutrition |
Vitamins |
Toxicology |
Applications of Chemistry |
Consumer Chemistry
Two spectrophotometric experiments with alpha-chymotrypsin  Kantrowitz, Evan R.; Eisele, George
Two spectrophotometric experiments with alpha-chymotrypsin; the first examines the mechanism of chymotrypsin using the acridine dye proflavine, while the second illustrates the activation of chymotrupsinogen by trypsin.
Kantrowitz, Evan R.; Eisele, George J. Chem. Educ. 1975, 52, 410.
Spectroscopy |
Enzymes |
Catalysis |
Proteins / Peptides
Hemoglobin: A series of biochemistry experiments  Slabaugh, Michael R.; Carrasco, Charles R.
Lists a series of experiments using hemoglobin to introduce students to the chemistry of proteins.
Slabaugh, Michael R.; Carrasco, Charles R. J. Chem. Educ. 1975, 52, 67.
Proteins / Peptides |
Electrophoresis |
Chromatography
Iron metabolism in the rat: An experiment in biochemistry  Paul, K. G.; Stigbrand, T.
Students isolate six iron-containing proteins (including hemoglobin, cytochrome C, myoglobin, and ferritin) from rats that have been injected with iron 59 citrate.
Paul, K. G.; Stigbrand, T. J. Chem. Educ. 1970, 47, 722.
Metabolism |
Proteins / Peptides |
Isotopes
Hormone structure and biological activity: Biochemical studies of three pituitary hormones  Papkoff, Harold; Li, Choh Hao
Presents results of biochemical studies examining growth hormone, adrenocorticotropin, and melanocyte stimulating hormone.
Papkoff, Harold; Li, Choh Hao J. Chem. Educ. 1966, 43, 41.
Hormones |
Proteins / Peptides |
Amino Acids
A Chemical Approach to Food and Nutrition (Fox, Brian A.; Cameron, Allan G.)  Watts, Betty M.

Watts, Betty M. J. Chem. Educ. 1962, 39, A422.
Food Science |
Nutrition
C. J. M. Mehu, a forgotten man of science  McCollum, E. V.
Traces the life and contributions to protein chemistry made by C. J. M. Mehu.
McCollum, E. V. J. Chem. Educ. 1956, 33, 507.
Proteins / Peptides
The molecular weight of a protein: A student determination of osmotic pressure  Peterson, Sigfred
With rather modest demands on equipment, time, and technique, this experiment gives for gelatin solutions molecular weights that, although not precise, agree in order of magnitude with each other and reported values.
Peterson, Sigfred J. Chem. Educ. 1951, 28, 486.
Molecular Properties / Structure |
Proteins / Peptides