| Journal Articles: 9 results |
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A-DNA and B-DNA: Comparing Their Historical X-ray Fiber Diffraction Images Amand A. Lucas
This paper provides a comparative explanation of the structural content of the diffraction diagrams of A-DNA and B-DNA that facilitated the discovery of the double-helical structure of DNA by Watson and Crick in 1953. This analysis is supported a method that simulates both A-DNA and B-DNA X-ray images optically.
Lucas, Amand A. J. Chem. Educ. 2008, 85, 737.
Biophysical Chemistry |
Conformational Analysis |
Crystals / Crystallography |
X-ray Crystallography |
Nucleic Acids / DNA / RNA
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Probing Changes in the Conformation of tRNAPhe: An Integrated Biochemistry Laboratory Course Sarah R. Kirk, Todd P. Silverstein, Karen L. McFarlane Holman, and Buck L. H. Taylor
This guided-inquiry, laboratory course focuses on the biomolecule tRNAPhe and combines biochemistry with elements of bioorganic and bioinorganic chemistry. Throughout the semester students work collaboratively to study tRNAPhe structure and the influence of ligand binding under a variety of conditions using several analytical techniques.
Kirk, Sarah R.; Silverstein, Todd P.; Holman, Karen L. McFarlane; Taylor, Buck L. H. J. Chem. Educ. 2008, 85, 666.
Bioinorganic Chemistry |
Bioorganic Chemistry |
Biophysical Chemistry |
Electrophoresis |
Fluorescence Spectroscopy |
UV-Vis Spectroscopy |
Student-Centered Learning |
Nucleic Acids / DNA / RNA
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Using Physical Models of Biomolecular Structures To Teach Concepts of Biochemical Structure and Structure Depiction in the Introductory Chemistry Laboratory Gordon A. Bain, John Yi, Mithra Beikmohamadi, Timothy M. Herman, and Michael A. Patrick
Custom-made physical models of alpha-helices and beta-sheets, the zinc finger moiety, beta-globin, and green fluorescent protein are used to introduce students in first-year chemistry to the primary, secondary, and tertiary structure of proteins.
Bain, Gordon A.; Yi, John; Beikmohamadi, Mithra; Herman, Timothy M.; Patrick, Michael A. J. Chem. Educ. 2006, 83, 1322.
Amino Acids |
Proteins / Peptides |
Molecular Modeling |
Molecular Properties / Structure |
Nucleic Acids / DNA / RNA
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Glycosyltransferases A and B: Four Critical Amino Acids Determine Blood Type Natisha L. Rose, Monica M. Palcic, and Stephen V. Evans
Human A, B, and O blood type is determined by the presence or absence of distinct carbohydrate structures on red blood cells. In this review the chemistry of the blood group ABO system and the role of glycosyltransferase A, glycosyltransferase B, and the four amino acids critical to determining blood group status are discussed.
Rose, Natisha L.; Palcic, Monica M.; Evans, Stephen V. J. Chem. Educ. 2005, 82, 1846.
Carbohydrates |
Enzymes |
Kinetics |
Bioorganic Chemistry |
Crystals / Crystallography |
Molecular Biology |
X-ray Crystallography |
Amino Acids
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Testing for Ultraviolet Toxicity Using Fungi Marcelo Vital and Patricia Esperón
In this article, we describe a laboratory project quantifying the effects of UV radiation on a suspension of living cells. This laboratory experiment was designed for undergraduate students in biochemistry. The experiment was carried out employing different fungi strains, changing the dosage of radiation, the distance to the UV source, and the exposure period to the UV radiation. The quantity of viable spores was calculated and the doseresponse curves were drawn. Students were able to prove that 100% of mortality was achieved with the longest time of exposure.
Vital, Marcelo; Esperón, Patricia. J. Chem. Educ. 2005, 82, 926.
Nucleic Acids / DNA / RNA |
UV-Vis Spectroscopy |
Medicinal Chemistry |
Molecular Biology |
Photochemistry
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Analysis of DAPI and SYBR Green I as Alternatives to Ethidium Bromide for Nucleic Acid Staining in Agarose Gel Electrophoresis Kevin M. Bourzac, Lori J. LaVine, and Margaret S. Rice
DAPI and SYBR Green I are good staining alternatives to ethidium bromide in that they have similar sensitivity and are both easy to use. SYBR Green I is more expensive than EB or DAPI; however, the limited safety data suggest that SYBR Green I is the safest stain.
Bourzac, Kevin M.; LaVine, Lori J.; Rice, Margaret S. J. Chem. Educ. 2003, 80, 1292.
Dyes / Pigments |
Electrophoresis |
Nucleic Acids / DNA / RNA
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An Attack on the AIDS Virus: Inhibition of the HIV-1 Protease. New Drug Development Based on the Structure and Activity of the Protease and its Role in the Replication and Maturation of the Virus Volker, Eugene J.
Article shows how research into the biochemistry of HIV may lead to a method for controlling its growth and maturation. This research illustrates some basic enzyme chemistry and holds student appeal due to the tie into current affairs.
Volker, Eugene J. J. Chem. Educ. 1993, 70, 3.
Bioorganic Chemistry |
Proteins / Peptides |
Medicinal Chemistry |
Molecular Biology
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Receptor G protein effector: The design of a biochemical switchboard Ross, Elliott M.
The author describes in detail how biochemical systems function chemically and how they are physically arrayed in the cell membrane.
Ross, Elliott M. J. Chem. Educ. 1988, 65, 937.
Enrichment / Review Materials |
Hormones |
Metabolism |
Molecular Biology |
Enzymes |
Proteins / Peptides |
Toxicology |
Bioenergetics
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The Molecular Basis of Evolution (Anfinsen, Christian B.) Edsall, John T.
Edsall, John T. J. Chem. Educ. 1960, 37, 107.
Molecular Biology
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