TIGER

Journal Articles: 8 results
Fluorescence Spectroscopy of tRNAPhe Y Base in the Presence of Mg2+ and Small Molecule Ligands  Sarah R. Kirk, Todd P. Silverstein, and Karen L. McFarlane Holman
This article describes a set of experiments in which ligands (e.g., spermine, neomycin B, magnesium) are titrated into tRNAPhe solution and fluorescence spectroscopy is used to draw conclusions on how different binding modes affect tRNAPhe tertiary structure.
Kirk, Sarah R.; Silverstein, Todd P.; Holman, Karen L. McFarlane. J. Chem. Educ. 2008, 85, 678.
Bioinorganic Chemistry |
Bioorganic Chemistry |
Biophysical Chemistry |
Fluorescence Spectroscopy |
Nucleic Acids / DNA / RNA
Metal-Catalyzed Cleavage of tRNAPhe  Sarah R. Kirk, Todd P. Silverstein, and Karen L. McFarlane Holman
This article describes a set of experiments in which students study tRNAPhe conformational changes induced by ligand binding using gel electrophoresis to determine how metal-catalyzed hydrolysis is affected by parameters such as tRNAPhe structure, ligand charge, and the chemistry of the metal ion complexes involved.
Kirk, Sarah R.; Silverstein, Todd P.; Holman, Karen L. McFarlane. J. Chem. Educ. 2008, 85, 676.
Bioinorganic Chemistry |
Bioorganic Chemistry |
Electrophoresis |
Nucleic Acids / DNA / RNA
UV Thermal Melting Curves of tRNAPhe in the Presence of Ligands  Sarah R. Kirk, Todd P. Silverstein, and Karen L. McFarlane Holman
This article describes a set of experiments in which the thermal unfolding of tRNAPhe is studied with UVĀvis spectrophotometry. Students examine the effects of magnesium and small molecule ligands on tRNAPhe conformational stability and draw conclusions about tRNAPhe ligand binding modes and the impact of various types of ligands on tRNA structure.
Kirk, Sarah R.; Silverstein, Todd P.; Holman, Karen L. McFarlane. J. Chem. Educ. 2008, 85, 674.
Bioinorganic Chemistry |
Bioorganic Chemistry |
Biophysical Chemistry |
Thermal Analysis |
UV-Vis Spectroscopy |
Nucleic Acids / DNA / RNA
Probing Changes in the Conformation of tRNAPhe: An Integrated Biochemistry Laboratory Course  Sarah R. Kirk, Todd P. Silverstein, Karen L. McFarlane Holman, and Buck L. H. Taylor
This guided-inquiry, laboratory course focuses on the biomolecule tRNAPhe and combines biochemistry with elements of bioorganic and bioinorganic chemistry. Throughout the semester students work collaboratively to study tRNAPhe structure and the influence of ligand binding under a variety of conditions using several analytical techniques.
Kirk, Sarah R.; Silverstein, Todd P.; Holman, Karen L. McFarlane; Taylor, Buck L. H. J. Chem. Educ. 2008, 85, 666.
Bioinorganic Chemistry |
Bioorganic Chemistry |
Biophysical Chemistry |
Electrophoresis |
Fluorescence Spectroscopy |
UV-Vis Spectroscopy |
Student-Centered Learning |
Nucleic Acids / DNA / RNA
Genetic Code Wordsearch  Terry L. Helser
Wordsearch puzzle containing 30 words that describe the nucleotide sequences used to produce proteins.
Helser, Terry L. J. Chem. Educ. 2003, 80, 417.
Biotechnology |
Nomenclature / Units / Symbols |
Enrichment / Review Materials |
Nucleic Acids / DNA / RNA
The conversion of chemical energy: Part 2. Biochemical examples  Wink, Donald J.
Biological systems regulate energy transfer reactions through enzymes that permit a spontaneous reaction to go faster through a mechanism that also accomplishes work instead of, or in addition to, releasing energy as heat.
Wink, Donald J. J. Chem. Educ. 1992, 69, 264.
Enzymes |
Bioorganic Chemistry |
Bioenergetics
Binding energy and enzymatic catalysis  Hansen, David E.; Raines, Ronald T.
The authors discuss the fundamental role that the favorable free energy of binding of the rate-determining transition state plays in catalysis and review the principle that many catalytic factors are realized by the use of this binding energy.
Hansen, David E.; Raines, Ronald T. J. Chem. Educ. 1990, 67, 483.
Enzymes |
Catalysis |
Thermodynamics
RNA's as catalysts: A new class on enzymes  McCorkle, George M.; Altman, Sidney
Analysis of two RNA's that act as enzymes, upsetting the long-held position that all enzymes are proteins.
McCorkle, George M.; Altman, Sidney J. Chem. Educ. 1987, 64, 221.
Catalysis |
Enzymes