Journal Articles: 19 results
Probing Changes in the Conformation of tRNAPhe: An Integrated Biochemistry Laboratory Course  Sarah R. Kirk, Todd P. Silverstein, Karen L. McFarlane Holman, and Buck L. H. Taylor
This guided-inquiry, laboratory course focuses on the biomolecule tRNAPhe and combines biochemistry with elements of bioorganic and bioinorganic chemistry. Throughout the semester students work collaboratively to study tRNAPhe structure and the influence of ligand binding under a variety of conditions using several analytical techniques.
Kirk, Sarah R.; Silverstein, Todd P.; Holman, Karen L. McFarlane; Taylor, Buck L. H. J. Chem. Educ. 2008, 85, 666.
Bioinorganic Chemistry |
Bioorganic Chemistry |
Biophysical Chemistry |
Electrophoresis |
Fluorescence Spectroscopy |
UV-Vis Spectroscopy |
Student-Centered Learning |
Nucleic Acids / DNA / RNA
Amino Acid Complementarity: A Biochemical Exemplar of Stoichiometry for General and Health Sciences Chemistry  Ed Vitz
Calculations demonstrating amino acid complementarity are presented as an interesting application of stoichiometry. Food proteins are said to have complementary amino acids when the proteins combine to provide amino acids in the proper stoichiometric ratios to synthesize human protein. Implications for vegetarian diet, efficiency of food production, and diet adaptations in various cultures are explored briefly.
Vitz, Ed. J. Chem. Educ. 2005, 82, 1013.
Amino Acids |
Proteins / Peptides |
Stoichiometry |
Food Science
Nonclassical or Reactivation Chain Polymerization: A General Scheme of Polymerization  Yen Wei
A new integrated model of polymerization is presented: the nonclassical or reactivation chain polymerization. In this model polymer growth involves chemical, physical, or biological reactivation of a dormant chain and subsequent reaction of the reactivated chain with incoming monomers to yield a new dormant chain of higher molecular weight.
Wei, Yen. J. Chem. Educ. 2001, 78, 551.
Materials Science |
Mechanisms of Reactions |
Proteins / Peptides |
Bioorganic Chemistry: Peptides and Proteins (edited by Sidney M. Hecht)  Spencer Anthony-Cahill
The second volume in the Bioorganic Chemistry series edited by Sidney Hecht is an outstanding addition to the collections of all scientists who teach and/or do research in the field of protein chemistry. The coverage of current research is up to date and thus the book is of great relevance to all chemists with interest in proteins, not just to academicians.
Anthony-Cahill, Spencer. J. Chem. Educ. 1999, 76, 905.
Bioorganic Chemistry |
Proteins / Peptides
Recombinant Green Fluorescent Protein Isoforms: Exercises To Integrate Molecular Biology, Biochemistry, and Biophysical Chemistry  Barry W. Hicks
Using common techniques of molecular biology, students transfect Escherichia coli with cloned DNA (cDNA) plasmids encoding for various isoforms of the green fluorescent protein (GFP) from the jellyfish Aequorea victoria. The different GFP isoforms can then be purified and characterized.
Hicks, Barry W. J. Chem. Educ. 1999, 76, 409.
Proteins / Peptides |
Photochemistry |
Molecular Properties / Structure |
Biotechnology |
Biophysical Chemistry
Guidebook to Protein Toxins and Their Use in Cell Biology (edited by R. Rappuoli and C. Montecucco)  Steven John Pernecky
The authors have concisely compiled structure, function, and toxicity data for 78 protein toxins in a unique, accessible format that allows cell biologists to learn how protein toxins have aided in the dissection of cellular processes.
Pernecky, Steven John. J. Chem. Educ. 1999, 76, 34.
Proteins / Peptides |
The Synthesis of Proteins-A Simple Experiment To Show the Procedures and Problems of Using Radioisotopes in Biochemical Studies  David M. Hawcroft
The experiment described in this paper uses simple techniques to illustrate the procedures involved in working with radioisotopes when following a simplified metabolic pathway. Safety considerations are discussed and a list of safety rules is provided, but the experiment itself uses very low levels of a weak beta-emitting isotope (tritium).
Hawcroft, David M. J. Chem. Educ. 1996, 73, 1030.
Proteins / Peptides |
Synthesis |
Nuclear / Radiochemistry
An Analysis of the Involvement of Purine Ribonucleotides in Eukaryotic Protein Synthesis: An Undergraduate Biochemistry Laboratory Experiment  T. Glen Lawson
Experimental procedure in which students can study directly the chemical mechanisms involved in the synthesis of proteins by cellular messenger RNA translation systems.
Lawson, T. Glen. J. Chem. Educ. 1995, 72, 1041.
Proteins / Peptides |
Nucleic acid structure and synthesis (Parish, J.H.)  Parker, Keith K.
A review of a software program designed to introduce current understanding of DNA and RNA structure and the dynamic processes of replication and transcription.
Parker, Keith K. J. Chem. Educ. 1988, 65, A298.
Molecular Biology
Energetics of polymerization: A contribution to an understanding of protein synthesis  Friedmann, Herbert C.
The author presents an approach to protein synthesis that clarifies some of the conundrums posed by a complex system.
Friedmann, Herbert C. J. Chem. Educ. 1986, 63, 678.
Proteins / Peptides |
Synthesis |
Perspectives in biochemistry: Methods for DNA sequencing  Wood, Anne T.
Describes how DNA is sequenced.
Wood, Anne T. J. Chem. Educ. 1984, 61, 886.
Proteins / Peptides |
Biotechnology |
The determination of vitamin D-dependent calcium binding protein in chick intestine: An undergraduate biochemistry laboratory experiment  Lessard, George M.
An experiment inducing rickets in chicks and correlating the disease to a reduction in vitamin D-dependent calcium binding protein demonstrates hormone induction, protein isolation, and radioisotope methodology.
Lessard, George M. J. Chem. Educ. 1980, 57, 674.
Vitamins |
Proteins / Peptides |
Nuclear / Radiochemistry |
Macromolecular solutions as an integral part of beginning physical chemistry  Krause, Sonja
The author discusses macromolecules in solution and to some of the topics from beginning courses in physical chemistry into which this subject should be integrated. The examples given here are not exhaustive, but they can be easily integrated into beginning courses.
Krause, Sonja J. Chem. Educ. 1978, 55, 174.
Solutions / Solvents |
Aqueous Solution Chemistry |
Synthesis |
Biophysical Chemistry |
Molecular Biology
Biochemical consequences of lipid peroxidation  Bland, Jeffrey
The mechanism of formation of lipid peroxides and their biochemical roles in observed changes in cellular proteins, enzymes, and nucleotides, lipofusion and period pigment formation, cellular aging, membrane alteration, crosslinking of collagen, and drug/chemical-induced pathologies constitute the focus of this report.
Bland, Jeffrey J. Chem. Educ. 1978, 55, 151.
Drugs / Pharmaceuticals |
Lipids |
Proteins / Peptides |
Enzymes |
Transport Properties |
Medicinal Chemistry
Non-covalent interactions: Key to biological flexibility and specificity  Frieden, Earl
Summarizes the types of non-covalent interactions found among biomolecules and how they facilitate the function of antibodies, hormones, and hemoglobin.
Frieden, Earl J. Chem. Educ. 1975, 52, 754.
Noncovalent Interactions |
Hydrogen Bonding |
Water / Water Chemistry |
Proteins / Peptides |
Amino Acids |
Molecular Properties / Structure |
Questions [and] Answers  Campbell, J. A.
Five questions requiring an application of basic chemical principles.
Campbell, J. A. J. Chem. Educ. 1972, 49, 269.
Enrichment / Review Materials |
Agricultural Chemistry |
Metabolism |
Calorimetry / Thermochemistry |
Proteins / Peptides
The significance of hydrogen bonds in biological structures  McClellan, A. L.
Examines RNA, DNA, some proteins, and enzyme (lysozyme), and yellow mosaic virus as examples to illustrate the significance of hydrogen bonds in biological structures.
McClellan, A. L. J. Chem. Educ. 1967, 44, 547.
Molecular Properties / Structure |
Hydrogen Bonding |
Noncovalent Interactions |
Proteins / Peptides |
Ribonucleic acid and protein synthesis  Roth, Jay S.
Examines the role of nucleic acids in protein synthesis.
Roth, Jay S. J. Chem. Educ. 1961, 38, 217.
Proteins / Peptides |
Synthesis |
Amino Acids
Immunochemistry: The chemistry of antigens and antibodies  Haurowitz, Felix
Examines the chemical aspects of antigens and antibodies.
Haurowitz, Felix J. Chem. Educ. 1955, 32, 615.
Medicinal Chemistry |
Proteins / Peptides |
Molecular Recognition