TIGER

Journal Articles: 10 results
A-DNA and B-DNA: Comparing Their Historical X-ray Fiber Diffraction Images  Amand A. Lucas
This paper provides a comparative explanation of the structural content of the diffraction diagrams of A-DNA and B-DNA that facilitated the discovery of the double-helical structure of DNA by Watson and Crick in 1953. This analysis is supported a method that simulates both A-DNA and B-DNA X-ray images optically.
Lucas, Amand A. J. Chem. Educ. 2008, 85, 737.
Biophysical Chemistry |
Conformational Analysis |
Crystals / Crystallography |
X-ray Crystallography |
Nucleic Acids / DNA / RNA
X-ray Diffraction and the Discovery of the Structure of DNA  David T. Crouse
Describes a step-by-step method of teaching the X-ray diffraction analysis of DNA using the approach employed by Watson, Crick, Wilkins, Franklin, and Gosling. Excerpts of their original articles describe the obstacles encountered by these researchers, the contributions made by each, and the degree to which X-ray diffraction aided their determination of DNA's structure.
Crouse, David T. J. Chem. Educ. 2007, 84, 803.
Biophysical Chemistry |
Crystals / Crystallography |
X-ray Crystallography |
Molecular Properties / Structure |
Molecular Modeling |
Nucleic Acids / DNA / RNA
Using Physical Models of Biomolecular Structures To Teach Concepts of Biochemical Structure and Structure Depiction in the Introductory Chemistry Laboratory  Gordon A. Bain, John Yi, Mithra Beikmohamadi, Timothy M. Herman, and Michael A. Patrick
Custom-made physical models of alpha-helices and beta-sheets, the zinc finger moiety, beta-globin, and green fluorescent protein are used to introduce students in first-year chemistry to the primary, secondary, and tertiary structure of proteins.
Bain, Gordon A.; Yi, John; Beikmohamadi, Mithra; Herman, Timothy M.; Patrick, Michael A. J. Chem. Educ. 2006, 83, 1322.
Amino Acids |
Proteins / Peptides |
Molecular Modeling |
Molecular Properties / Structure |
Nucleic Acids / DNA / RNA
A Unique Demonstration Model of DNA  Jonathan P. L. Cox
Describes a physical demonstration model of DNA for the classroom. The model comprises two types of building blocks that can be put together rapidly to produce an abstract structure that portrays several of the gross architectural features of idealized B-DNA.
Cox, Jonathan P. L. J. Chem. Educ. 2006, 83, 1319.
Molecular Biology |
Molecular Properties / Structure |
Student-Centered Learning |
Nucleic Acids / DNA / RNA
Label-Free Detection of DNA Hybridization by Cyclic Voltammetry. An Advanced Undergraduate Analytical Chemistry Laboratory Experiment  Temitope Aiyejorun, Janusz Kowalik, Jiri Janata, and Mira Josowicz
Describes a set of experiments designed to explore several concepts relevant to electropolymerization of intrinsically conducting polymers, their use as an electrically controlled ion-exchanger of chloride ions, and their ability to bind oligonucleotides through a noncovalent interaction with magnesium ions used as a linker between the polymer and the DNA strand.
Aiyejorun, Temitope; Kowalik, Janusz; Janata, Jiri; Josowicz, Mira. J. Chem. Educ. 2006, 83, 1208.
Biophysical Chemistry |
Electrochemistry |
Kinetics |
Undergraduate Research |
Nucleic Acids / DNA / RNA
A 3D Model of Double-Helical DNA Showing Variable Chemical Details  Susan G. Cady
A 3D double-helical DNA model, made by placing beads on a wire and stringing beads through holes in plastic canvas, is described. Suggestions are given to enhance the basic helical frame to show the shapes and sizes of the nitrogenous base rings, 3' and 5' chain termini, and base pair hydrogen bonding. Students can incorporate random or real gene sequence data into their models.
Cady, Susan G. J. Chem. Educ. 2005, 82, 79.
Biotechnology |
Molecular Properties / Structure |
Molecular Modeling |
Nucleic Acids / DNA / RNA
DNA Topology Analysis in the Undergraduate Biochemistry Laboratory  Michael V. Keck
A set of complementary experiments is described which are useful in teaching the principles of DNA topology to undergraduate biochemistry students.
Keck, Michael V. J. Chem. Educ. 2000, 77, 1471.
Drugs / Pharmaceuticals |
Electrophoresis |
Molecular Properties / Structure |
Nucleic Acids / DNA / RNA
Thermodynamics of DNA Duplex Formation: A Biophysical Chemistry Laboratory Experiment  Kathleen P. Howard
The goal of this biophysical experiment is to measure thermodynamic properties of a short DNA duplex by melting the ordered native structure (duplex) into the disordered, denatured state (single strands) while monitoring the transition using ultraviolet spectrophotometry.
Howard, Kathleen P. J. Chem. Educ. 2000, 77, 1469.
Biophysical Chemistry |
Thermodynamics |
UV-Vis Spectroscopy |
Nucleic Acids / DNA / RNA
Liver and Onions: DNA Extraction from Animal and Plant Tissues  Karen J. Nordell, Anne-Marie L. Jackelen, S. Michael Condren, George C. Lisensky, and Arthur B. Ellis*
This activity, which allows students to extract DNA from plant and animal cells, serves as a spectacular example of the complexity of biochemical structure and function and fits well with a discussion of nucleic acids, hydrogen bonding, genetic coding, and heredity. DNA extraction can also be used in conjunction with a discussion of polymers and their properties.
Nordell, Karen J.; Jackelen, Anne-Marie L.; Condren, S. Michael; Lisensky, George C.; Ellis, Arthur B. J. Chem. Educ. 1999, 76, 400A.
Hydrogen Bonding |
Molecular Properties / Structure |
Nucleic Acids / DNA / RNA
Revealing the Backbone Structure of B-DNA from Laser Optical Simulations of Its X-ray Diffraction Diagram  A. A. Lucas, Ph. Lambin, R. Mairesse, and M. Mathot
A visible laser and a set of nine specially designed diffraction gratings held on a single slide have been used to build up progressively a classroom optical simulation of the diffraction of X-rays by a B-DNA fiber. The nine optical transforms allow understanding, without recourse to helical diffraction theory, of how the prominent features of the observed diffraction pattern arise from each of the structural parameters of the DNA backbone.
Lucas, A. A.; Lambin, Ph.; Mairesse, R.; Mathot, M. J. Chem. Educ. 1999, 76, 378.
Crystals / Crystallography |
X-ray Crystallography |
Molecular Properties / Structure |
Nucleic Acids / DNA / RNA