TIGER

Journal Articles: 6 results
A-DNA and B-DNA: Comparing Their Historical X-ray Fiber Diffraction Images  Amand A. Lucas
This paper provides a comparative explanation of the structural content of the diffraction diagrams of A-DNA and B-DNA that facilitated the discovery of the double-helical structure of DNA by Watson and Crick in 1953. This analysis is supported a method that simulates both A-DNA and B-DNA X-ray images optically.
Lucas, Amand A. J. Chem. Educ. 2008, 85, 737.
Biophysical Chemistry |
Conformational Analysis |
Crystals / Crystallography |
X-ray Crystallography |
Nucleic Acids / DNA / RNA
Quantitative Determination of DNA–Ligand Binding Using Fluorescence Spectroscopy  Eamonn F. Healy
The experiment described here measures the DAPI (4',6-diamidino-2-phenylindole) binding constant for calf-thymus DNA using fluorescence measurements and a modified Scatchard analysis. Student can estimate DAPI occupancy of the minor groove in DNA by calculating the number of nucleotides per bound ligand.
Healy, Eamonn F. J. Chem. Educ. 2007, 84, 1304.
Bioanalytical Chemistry |
Drugs / Pharmaceuticals |
Fluorescence Spectroscopy |
Medicinal Chemistry |
Nucleic Acids / DNA / RNA
A 3D Model of Double-Helical DNA Showing Variable Chemical Details  Susan G. Cady
A 3D double-helical DNA model, made by placing beads on a wire and stringing beads through holes in plastic canvas, is described. Suggestions are given to enhance the basic helical frame to show the shapes and sizes of the nitrogenous base rings, 3' and 5' chain termini, and base pair hydrogen bonding. Students can incorporate random or real gene sequence data into their models.
Cady, Susan G. J. Chem. Educ. 2005, 82, 79.
Biotechnology |
Molecular Properties / Structure |
Molecular Modeling |
Nucleic Acids / DNA / RNA
Nucleic Acids: Structures, Properties, and Functions (by Victor A. Bloomfield, Donald M. Crothers, and Ignacio Tinoco Jr.; with contributions from John E. Hearst, David E. Wemmer, Peter A. Kollman, and Douglas H. Turner)  John H. Shibata
A single volume that contains a comprehensive and detailed account of the structures and physical properties of nucleic acids.
Shibata, John H. J. Chem. Educ. 2001, 78, 314.
Molecular Biology |
Molecular Properties / Structure |
Nucleic Acids / DNA / RNA
Monitoring Hammerhead Ribozyme-Catalyzed Cleavage with a Fluorescein-Labeled Substrate: Effects of Magnesium Ions and Antibiotic Inhibitors. A Biochemistry Laboratory: Part 2  Christine S. Chow, Smita Somne, and Beatriz Llano-Sotelo
An experiment is presented that demonstrates current techniques in modern RNA research and introduces a method for nonradioactive monitoring of RNA reactions. The laboratory involves the study of hammerhead ribozyme activity and the influence of metal ions and antibiotics on these important RNA-based reactions.
Chow, Christine S.; Somne, Smita; Sotelo, Beatriz Llano. J. Chem. Educ. 1999, 76, 651.
Bioorganic Chemistry |
Electrophoresis |
Enzymes |
Bioinorganic Chemistry |
Nucleic Acids / DNA / RNA
Synthesis and Purification of a Hammerhead Ribozyme and a Fluorescein-Labeled RNA Substrate. A Biochemistry Laboratory: Part 1  Christine S. Chow and Smita Somne
The applications of in vitro transcription and chemical synthesis of RNA are discussed. This laboratory describes the in vitro synthesis of a 38-nucleotide hammerhead ribozyme and the synthesis of a 17-nucleotide fluorescein-labeled RNA substrate by using standard phosphoramidite methodologies, two widely used methods in modern RNA research.
Chow, Christine S.; Somne, Smita. J. Chem. Educ. 1999, 76, 648.
Bioorganic Chemistry |
Electrophoresis |
Enzymes |
Bioinorganic Chemistry |
Nucleic Acids / DNA / RNA