TIGER

Journal Articles: 21 results
Frank Westheimer's Early Demonstration of Enzymatic Specificity  Addison Ault
Reviews one of the most significant accomplishments of one of the most respected chemists of the 20th century┬Ła series of stereospecific enzymatic oxidation and reduction experiments that led chemists to recognize enantiotopic and diastereotopic relationships of atoms, or groups of atoms, within molecules.
Ault, Addison. J. Chem. Educ. 2008, 85, 1246.
Asymmetric Synthesis |
Bioorganic Chemistry |
Catalysis |
Chirality / Optical Activity |
Enantiomers |
Enzymes |
Isotopes |
Nucleophilic Substitution |
Oxidation / Reduction |
Stereochemistry
Glycosyltransferases A and B: Four Critical Amino Acids Determine Blood Type  Natisha L. Rose, Monica M. Palcic, and Stephen V. Evans
Human A, B, and O blood type is determined by the presence or absence of distinct carbohydrate structures on red blood cells. In this review the chemistry of the blood group ABO system and the role of glycosyltransferase A, glycosyltransferase B, and the four amino acids critical to determining blood group status are discussed.
Rose, Natisha L.; Palcic, Monica M.; Evans, Stephen V. J. Chem. Educ. 2005, 82, 1846.
Carbohydrates |
Enzymes |
Kinetics |
Bioorganic Chemistry |
Crystals / Crystallography |
Molecular Biology |
X-ray Crystallography |
Amino Acids
Chemical Modification of Papain and Subtilisin: An Active Site Comparison. An Undergraduate Biochemistry Experiment   Mireille St-Vincent and Michael Dickman
This experiment demonstrates the specific chemistry of cysteine and serine residues in the active sites of papain and subtilisin.
St-Vincent, Mireille; Dickman, Michael. J. Chem. Educ. 2004, 81, 1048.
Amino Acids |
Bioorganic Chemistry |
Enzymes
"Chiral Acetate": The Preparation, Analysis, and Applications of Chiral Acetic Acid  Addison Ault
Production of chiral acetic acid using deuterium and tritium and its application to understanding stereochemistry and the specificity of enzymatic reactions.
Ault, Addison. J. Chem. Educ. 2003, 80, 333.
Chirality / Optical Activity |
Enzymes |
Isotopes |
Synthesis |
Stereochemistry |
Enrichment / Review Materials |
Carboxylic Acids |
Enantiomers |
Reactions |
Mechanisms of Reactions
Factors Affecting Reaction Kinetics of Glucose Oxidase  Kristin A. Johnson
Demonstration based on a biochemical kinetics experiment in which the rate of reaction varies with the enzyme concentration, substrate concentration, substrate used in the reaction, and temperature.
Johnson, Kristin A. J. Chem. Educ. 2002, 79, 74.
Enzymes |
Kinetics |
Proteins / Peptides |
Carbohydrates |
Catalysis |
Rate Law
A Modification of a Lactase Experiment by Use of Commercial Test Strips  Tammy J. Melton
Using urinalysis test strips to detect the presence of glucose.
Melton, Tammy J. J. Chem. Educ. 2001, 78, 1243.
Carbohydrates |
Catalysis |
Drugs / Pharmaceuticals |
Enzymes |
Nonmajor Courses |
Qualitative Analysis |
Laboratory Equipment / Apparatus
Bioanalytical Experiments for the Undergraduate Laboratory: Monitoring Glucose in Sports Drinks  J. Justin Gooding, Wenrong Yang, and Manihar Situmorang
Introducing students to the techniques of bioanalytical chemistry by using a solution-based enzyme assay and an enzyme electrode for the analysis of glucose concentrations in sports drinks.
Gooding, J. Justin; Yang, Wenrong; Situmorang, Manihar. J. Chem. Educ. 2001, 78, 788.
Electrochemistry |
Enzymes |
Food Science |
Quantitative Analysis |
Applications of Chemistry |
Carbohydrates
Enzyme-Linked Antibodies: A Laboratory Introduction to the ELISA  Gretchen L. Anderson and Leo A. McNellis
A fast and economical laboratory exercise is presented that qualitatively demonstrates the power of enzyme-linked antibodies to detect a specific antigen. Although ELISAs are commonly used in disease diagnosis in clinical settings, this application uses biotin, covalently attached to albumin, to take advantage of readily available reagents and avoids problems associated with potentially pathogenic antigens.
Anderson, Gretchen L.; McNellis, Leo A. J. Chem. Educ. 1998, 75, 1275.
Enzymes |
Nonmajor Courses |
Medicinal Chemistry
Working with Enzymes - Where Is Lactose Digested? An Enzyme Assay for Nutritional Biochemistry Laboratories  Sandi R. Pope, Tonya D. Tolleson, R. Jill Williams, Russell D. Underhill, and S. Todd Deal
An enzyme assay utilizing lactase enzyme from crushed LactAid tablets and a 5% lactose solution ("synthetic milk"). In the experiment, the students assay the activity of the enzyme on the "synthetic milk" at pHs of approximately 1, 6, and 8 with the stated goal of determining where lactose functions in the digestive tract. The activity of the lactase may be followed chromatographically or spectrophotometrically.
Pope, Sandi R.; Tolleson, Tonya D.; Williams, R. Jill; Underhill, Russell D.; Deal, S. Todd. J. Chem. Educ. 1998, 75, 761.
Enzymes |
Carbohydrates |
Catalysis |
Chromatography |
Spectroscopy |
Nutrition
Kinetic Study of the Enzyme Urease from Dolichos biflorus  Natarajan, K. R.
Procedure for studying the enzyme urease to catalyze the hydrolysis of urea.
Natarajan, K. R. J. Chem. Educ. 1995, 72, 556.
Enzymes
Nucleophilic and Enzymic Catalysis of p-Nitrophenylacetate Hydrolysis  Head, Michael B.; Mistry, Kalpna S.; Ridings, Bernard J.; Smith, Christopher A.; Parker, Mark J.
Experimental procedure for determining the relative effectiveness of several amino acids and enzymes in catalyzing the hydrolysis of p-nitrophenylacetate; sample data and analysis included.
Head, Michael B.; Mistry, Kalpna S.; Ridings, Bernard J.; Smith, Christopher A.; Parker, Mark J. J. Chem. Educ. 1995, 72, 184.
Amino Acids |
Enzymes |
Proteins / Peptides |
Catalysis
Detoxifying enzymes and insect symbionts  Shen, Samuel K.; Dowd, Patrick F.
Enzymes and pathways for the detoxification of substances in a variety of organisms.
Shen, Samuel K.; Dowd, Patrick F. J. Chem. Educ. 1992, 69, 796.
Enzymes |
Toxicology
RNA's as catalysts: A new class on enzymes  McCorkle, George M.; Altman, Sidney
Analysis of two RNA's that act as enzymes, upsetting the long-held position that all enzymes are proteins.
McCorkle, George M.; Altman, Sidney J. Chem. Educ. 1987, 64, 221.
Catalysis |
Enzymes
The enzymatic resolution of aromatic amino acids  Sheardy, Riehard; Liotta, L.; Steinhart, E.; Champion, R.; Rinker, J.; Planutis, M.; Salinkas, J.; Boyer, T.; Carcanague, D.
This article presents an experiment that can demonstrate as many principles of steroisomersim as possible and is also efficient in terms of time and preparation.
Sheardy, Riehard; Liotta, L.; Steinhart, E.; Champion, R.; Rinker, J.; Planutis, M.; Salinkas, J.; Boyer, T.; Carcanague, D. J. Chem. Educ. 1986, 63, 646.
Stereochemistry |
Chirality / Optical Activity |
Enantiomers |
Aromatic Compounds |
Amino Acids |
Enzymes
A useful model for the "lock and key" analogy  Fenster, Ariel E.; Harpp, David N.; Schwarcz, Joseph A.
A model that nicely illustrates this principle is the "SOMA" puzzle cube.
Fenster, Ariel E.; Harpp, David N.; Schwarcz, Joseph A. J. Chem. Educ. 1984, 61, 967.
Molecular Modeling |
Molecular Properties / Structure |
Molecular Recognition |
Enzymes
New skeletal-space-filling models. A model of an enzyme active site  Clarke, Frank H.
Reviews the molecular modeling systems available for representing organic and biochemical structures; includes requirements and coordinates for a model of the alpha chymotrypsin active site.
Clarke, Frank H. J. Chem. Educ. 1977, 54, 230.
Molecular Properties / Structure |
Enzymes |
Molecular Modeling |
Molecular Recognition
Cycloamyloses  Bergeron, Raymond J.
Examines an unusual carbohydrate system of current interest in the hope of generating some enthusiasm for the topic.
Bergeron, Raymond J. J. Chem. Educ. 1977, 54, 204.
Carbohydrates |
Molecular Properties / Structure |
Thermodynamics |
Kinetics |
Enzymes |
Spectroscopy
Simple models for tough concepts  Cavagnol, Richard M.; Barnett, Thomas
One of the most challenging aspects of instructional interaction is the presentation of dynamic chemical concepts interaction is the presentation of dynamic chemical concepts in a way that is both believable and understandable. The authors have devised a series of models that are simple, inexpensive, and require very little time or skill to construct. They allow students to visualize a whole spectrum of phenomena from atomic structure to enzyme-substrate interactions.
Cavagnol, Richard M.; Barnett, Thomas J. Chem. Educ. 1976, 53, 643.
Enzymes |
Molecular Modeling |
Molecular Mechanics / Dynamics |
Atomic Properties / Structure |
Transport Properties
Interactions of enzymes and inhibitors  Baker, B. R.
Examines the kinetics and interactions of enzymes and inhibitors and considers specifically lactic dehydrogenase, dihydrofolic reductase, thymidine phosphorylate, guanase, and xanthine oxidase.
Baker, B. R. J. Chem. Educ. 1967, 44, 610.
Enzymes |
Catalysis |
Noncovalent Interactions |
Molecular Properties / Structure |
Molecular Recognition |
Hydrogen Bonding
Three-dimensional effects in biochemistry  Ingraham, Lloyd L.
Explores stereospecificity and stereoselectivity; rigidity requirements; steric effects; and stereospecificity when not required mechanistically.
Ingraham, Lloyd L. J. Chem. Educ. 1964, 41, 66.
Molecular Properties / Structure |
Catalysis |
Enzymes |
Molecular Recognition |
Mechanisms of Reactions |
Stereochemistry |
Chirality / Optical Activity |
Enantiomers
Papain as an enzyme catalyst in undergraduate organic chemistry  Abernethy, John Leo; Kientz, Marvin
A rather large number of papain-catalyzed reactions can be selected for use in undergraduate organic laboratory work.
Abernethy, John Leo; Kientz, Marvin J. Chem. Educ. 1959, 36, 582.
Enzymes |
Catalysis