| Journal Articles: 98 results |
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Molecules and Medicine (E. J. Corey, Barbara Czakó, and László Kürti) Robert E. Buntrock
Looking for a book on common drugs and pharmaceuticals? On diseases and medical conditions? On pharmacology? In addition, do you need some background in chemistry to handle all of this information? If you want all of this, and in addition want it under one cover, then this is the book for you.
Buntrock, Robert E. J. Chem. Educ. 2008, 85, 1495.
Bioorganic Chemistry |
Drugs / Pharmaceuticals |
Molecular Properties / Structure |
Proteins / Peptides |
Synthesis |
Toxicology
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OMLeT—An Alternative Approach to Learning Metabolism: Glycolysis and the TCA Cycle as an Example Charles M. Stevens, Dylan M. Silver, Brad Behm, Raymond J. Turner, and Michael G. Surette
Using PHP Hypertext Preprocessor scripting, the dynamic OMLeT (Online Metabolism Learning Tool) Web site is geared towards different learning styles and allows the student to process metabolic pathways (glycolysis and TCA cycle) via a user-defined approach.
Stevens, Charles M.; Silver, Dylan M.; Behm, Brad; Turner, Raymond J.; Surette, Michael G. J. Chem. Educ. 2007, 84, 2024.
Bioenergetics |
Enzymes |
Learning Theories |
Metabolism |
Proteins / Peptides
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The A1c Blood Test: An Illustration of Principles from General and Organic Chemistry Robert C. Kerber
The glycated hemoglobin blood test is a key measure of the effectiveness of glucose control in diabetics. The chemistry of glucose in the bloodstream, which underlies the test and its impact, provides an illustration of the importance of chemical equilibrium and kinetics to a major health problem.
Kerber, Robert C. . J. Chem. Educ. 2007, 84, 1541.
Applications of Chemistry |
Bioinorganic Chemistry |
Carbohydrates |
Mechanisms of Reactions |
Proteins / Peptides |
Bioorganic Chemistry
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Nature's Way To Make the Lantibiotics Heather A. Relyea and Wilfred A. van der Donk
This article focuses on one class of antimicrobial compounds, the lantibiotics, and discusses their biosynthetic pathways as well as their molecular mode of action. In the course of the review, the meaning of the terms regio-, chemo-, and stereoselectivity are discussed.
Relyea, Heather A.; van der Donk, Wilfred A. J. Chem. Educ. 2006, 83, 1769.
Applications of Chemistry |
Bioorganic Chemistry |
Biotechnology |
Biosynthesis |
Catalysis |
Drugs / Pharmaceuticals |
Proteins / Peptides
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Amino Acids William F. Coleman
The Featured Molecules this month are the 20 standard alpha-amino acids found in proteins. The molecules are presented in two formats, the neutral form and the ionized form found in solution at physiologic pH.
Coleman, William F. J. Chem. Educ. 2006, 83, 1103.
Amino Acids |
Proteins / Peptides |
Molecular Properties / Structure |
Molecular Modeling |
Molecular Mechanics / Dynamics
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Caught on Tape: Catalyst Recovery; Secondary Structure Switch; DNA-Based Chiral Catalysts Angela G. King
Common lab tape aids catalyst delivery and recovery, DNA lends its chirality to organic reaction, and a model for studying the transformation of ahelices to ?sheets.
King, Angela G. J. Chem. Educ. 2006, 83, 10.
Catalysis |
Chirality / Optical Activity |
Proteins / Peptides |
Molecular Modeling |
Molecular Properties / Structure
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A Green Polymerization of Aspartic Acid for the Undergraduate Organic Laboratory George D. Bennett
Based on a technology that won a Presidential Green Chemistry Challenge Award, this experiment involves the thermal polymerization of aspartic acid and subsequent hydrolysis to give sodium poly(aspartate). The procedure is suitable for introducing students to the important topic of polymers and for illustrating several of the principles of green chemistry.
Bennett, George D. J. Chem. Educ. 2005, 82, 1380.
Green Chemistry |
Synthesis |
Industrial Chemistry |
Natural Products |
Polymerization |
Proteins / Peptides
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A Supramolecular Approach to Medicinal Chemistry: Medicine Beyond the Molecule David K. Smith
This article emphasizes a conceptual view of medicinal chemistry, which has important implications for the future, as the supramolecular approach to medicinal-chemistry products outlined here is rapidly allowing nanotechnology to converge with medicine. In particular, this article discusses recent developments including the rational design of drugs such as Relenza and Tamiflu, the mode of action of vancomycin, and the mechanism by which bacteria develop resistance, drug delivery using cyclodextrins, and the importance of supramolecular chemistry in understanding protein aggregation diseases such as Alzheimer's and CreutzfieldJacob.
Smith, David K. J. Chem. Educ. 2005, 82, 393.
Drugs / Pharmaceuticals |
Noncovalent Interactions |
Medicinal Chemistry |
Nanotechnology |
Proteins / Peptides
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An SDS–PAGE Examination of Protein Quaternary Structure and Disulfide Bonding for a Biochemistry Laboratory Jennifer L. Powers, Carla S. Andrews, Caroline C. St. Antoine, Swapan S. Jain, and Vicky L. H. Bevilacqua
In this experiment, students use sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDSPAGE) and -mercaptoethanol (ME) to determine preliminary information about protein quaternary structure. This experiment gives students a better understanding of protein subunit composition and the forces responsible for the stability of quaternary structure. The experiment can be accomplished in one three-hour laboratory period.
Powers, Jennifer L.; Andrews, Carla S.; St. Antoine, Caroline C.; Jain, Swapan S.; Bevilacqua, Vicky L. H. J. Chem. Educ. 2005, 82, 93.
Bioanalytical Chemistry |
Electrophoresis |
Laboratory Equipment / Apparatus |
Proteins / Peptides
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Proposed Improvements to a Lab on Equilibrium Binding of Phenol Red to Protein Todd Silverstein
Many biochemistry courses incorporate a laboratory project published as experiment 3 in Modern Experimental Biochemistry. Boyer recommends that students calculate the fraction of bound dye using the areas of the two eluted peaks in the absorbance versus fraction number plot. This will only work if the dyes absorbance is identical for both the bound and the free form.
Silverstein, Todd. J. Chem. Educ. 2004, 81, 645.
Dyes / Pigments |
Chromatography |
Proteins / Peptides |
Equilibrium
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The Proteomics Stock Market Project. A Cross-Disciplinary Collaboration in Biochemistry and Business Education Heath Keller and James R. Cox
Students taking courses in different disciplines can work together to add unique elements to their educational experience. Stage I involved biochemistry students investigating the topic of proteomics and choosing companies for potential investment based only on scientific investigation. Marketing and management students completed Stage II and provided an investment analysis on the companies selected in Stage I.
Keller, Heath; Cox, James R. J. Chem. Educ. 2004, 81, 519.
Drugs / Pharmaceuticals |
Proteins / Peptides
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A Modular Laser Apparatus for Polarimetry, Nephelometry, and Fluorimetry in General Chemistry Scott A. Darveau, Jessica Mueller, April Vaverka, Cheri Barta, Anthony Fitch, Jessica Jurzenski, and Yvonne Gindt
We present an apparatus suitable for multiple uses in the general chemistry laboratory including polarimetry, fluorescence, and nephelometry. The open design of the instrument also decreases the chance that students will contract the "black-box syndrome" that seems to develop when using instruments that only provide the final data in an experiment without showing how the measurements are obtained.
Darveau, Scott A.; Mueller, Jessica; Vaverka, April; Barta, Cheri; Fitch, Anthony; Jurzenski, Jessica; Gindt, Yvonne. J. Chem. Educ. 2004, 81, 401.
Fluorescence Spectroscopy |
Kinetics |
Laboratory Equipment / Apparatus |
Lasers |
Spectroscopy |
Proteins / Peptides |
Water / Water Chemistry
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LC–MS of Metmyoglobin at pH = 2. Separation and Characterization of Apomyoglobin and Heme by ESI–MS and UV–Vis Helen Cleary Stynes, Araceli Layo, and Richard W. Smith
This article describes an experiment employing LCMS where metmyoglobin is denatured to apomyoglobin and heme in a mobile phase gradient of acetonitrile H2O with 0.1% trifluoroacetic acid. The apomyoglobin is separated from the heme group by reversed-phase chromatography.
Stynes, Helen Cleary; Layo, Araceli; Smith, Richard W. J. Chem. Educ. 2004, 81, 266.
Biotechnology |
Chromatography |
Instrumental Methods |
Mass Spectrometry |
Proteins / Peptides |
UV-Vis Spectroscopy
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A "Polypeptide Demonstrator" Addison Ault
I have used a telephone Handset Coil Cord as a simple and convenient model for the structure of a polypeptide.
Ault, Addison. J. Chem. Educ. 2004, 81, 196.
Proteins / Peptides |
Molecular Modeling |
Molecular Properties / Structure
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Some Like It Cold: A Computer-Based Laboratory Introduction to Sequence and Tertiary Structure Comparison of Cold-Adapted Lactate Dehydrogenases Using Bioinformatics Tools M. Sue Lowery and Leigh A. Plesniak
Students download sequences and structures from appropriate databases, create sequence alignments, and carry out molecular modeling exercises, and then form hypotheses about the mechanism of biochemical adaptation for function and stability. This laboratory is appropriate for biochemistry and molecular biology laboratory courses, special topics, and advanced biochemistry lecture courses, and can be adapted for honors high school programs.
Lowery, M. Sue; Plesniak, Leigh A. J. Chem. Educ. 2003, 80, 1300.
Enzymes |
Molecular Modeling |
Proteins / Peptides |
Molecular Properties / Structure
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Protein Design Using Unnatural Amino Acids Basar Bilgiçer and Krishna Kumar
Using examples from the literature, this article describes the available methods for unnatural amino acid incorporation and highlights some recent applications including the design of hyperstable protein folds.
Bilgiçer, Basar; Kumar, Krishna. J. Chem. Educ. 2003, 80, 1275.
Amino Acids |
Bioorganic Chemistry |
Biotechnology |
Proteins / Peptides |
Synthesis |
Molecular Properties / Structure
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Milk as a Drug Analysis Medium: HPLC Determination of Isoniazid Enzo Sottofattori, Riccardo Raggio, and Olga Bruno
Using milk as a safer biological fluid for drug analysis; detecting isoniazid in cow's milk through HPLC.
Sottofattori, Enzo; Raggio, Riccardo; Bruno, Olga. J. Chem. Educ. 2003, 80, 547.
Chromatography |
Drugs / Pharmaceuticals |
Food Science |
Proteins / Peptides |
HPLC |
Quantitative Analysis |
Applications of Chemistry
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Identifying a Protein by MALDI–TOF Mass Spectrometry: An Experiment for the Undergraduate Laboratory Anne E. Counterman, Matthew S. Thompson, and David E. Clemmer
Experiment that requires students to use mass spectral data (MALDI-TOF) to find the identity of an unknown protein sample by performing a database search on the Internet.
Counterman, Anne E.; Thompson, Matthew S.; Clemmer, David E. J. Chem. Educ. 2003, 80, 177.
Mass Spectrometry |
Proteins / Peptides |
Instrumental Methods |
Qualitative Analysis
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Collaboration between Chemistry and Biology to Introduce Spectroscopy, Electrophoresis, and Molecular Biology as Tools for Biochemistry Vicky L. H. Bevilacqua, Jennifer L. Powers, Connie Tran, Swapan S. Jain, Reem Chabayta, Dale L. Vogelien, Ralph J. Rascati, Michelle Hall, and Kathleen Diehl
Program that integrates a variety of instrumental techniques across the biological and chemistry curricula, including biochemistry, plant physiology, genetics, and forensics.
Bevilacqua, Vicky L. H.; Powers, Jennifer L.; Vogelien, Dale L.; Rascati, Ralph J.; Hall, Michelle; Diehl, Kathleen; Tran, Connie; Jain, Swapan S.; Chabayta, Reem . J. Chem. Educ. 2002, 79, 1311.
Biotechnology |
Enzymes |
Forensic Chemistry |
Hormones |
Instrumental Methods |
Kinetics |
Plant Chemistry |
Proteins / Peptides |
UV-Vis Spectroscopy
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Entropy Loss of Hydroxyl Groups of Balanol upon Binding to Protein Kinase A Gergely Gidofalvi, Chung F. Wong, and J. Andrew McCammon
Summer project to give undergraduates hands-on experience in using several modern tools of molecular modeling for computer-aided drug design.
Gidofalvi, Gergely; Wong, Chung F.; McCammon, J. Andrew. J. Chem. Educ. 2002, 79, 1122.
Computational Chemistry |
Drugs / Pharmaceuticals |
Medicinal Chemistry |
Molecular Recognition |
Undergraduate Research |
Molecular Modeling |
Statistical Mechanics |
Thermodynamics |
Proteins / Peptides
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Factors Affecting Reaction Kinetics of Glucose Oxidase Kristin A. Johnson
Demonstration based on a biochemical kinetics experiment in which the rate of reaction varies with the enzyme concentration, substrate concentration, substrate used in the reaction, and temperature.
Johnson, Kristin A. J. Chem. Educ. 2002, 79, 74.
Enzymes |
Kinetics |
Proteins / Peptides |
Carbohydrates |
Catalysis |
Rate Law
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Blood-Chemistry Tutorials: Teaching Biological Applications of General Chemistry Material Rachel E. Casiday, Dewey Holten, Richard Krathen, and Regina F. Frey
Four, Web-based tutorials that deal with chemical processes in the blood and provide an integrated biological context for a variety of chemical concepts.
Casiday, Rachel E.; Holten, Dewey; Krathen, Richard; Frey, Regina F. J. Chem. Educ. 2001, 78, 1210.
Applications of Chemistry |
Medicinal Chemistry |
Proteins / Peptides |
Acids / Bases |
Equilibrium |
Molecular Properties / Structure
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Protein Structure Wordsearch Terry L. Helser
Puzzle with 37 names, terms, prefixes, and acronyms that describe protein structure.
Helser, Terry L. J. Chem. Educ. 2001, 78, 474.
Proteins / Peptides |
Nomenclature / Units / Symbols |
Molecular Properties / Structure
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The Chemical Adventures of Sherlock Holmes: The Shroud of Spartacus Thomas G. Waddell and Thomas R. Rybolt
A chemical mystery featuring Sherlock Holmes and Dr. Watson.
Waddell, Thomas G.; Rybolt, Thomas R. J. Chem. Educ. 2001, 78, 470.
Enrichment / Review Materials |
Forensic Chemistry |
Proteins / Peptides |
Quantitative Analysis
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An Inexpensive Gradient Maker for the Biochemistry Laboratory William H. Flurkey
A gradient maker for use in the biochemistry laboratory was constructed out of plastic graduated cylinders or other easily obtained plastic items. Assembly of the gradient makers requires little time or expense. Details on construction and operation are given.
Flurkey, Bill. J. Chem. Educ. 2000, 77, 1041.
Chromatography |
Laboratory Equipment / Apparatus |
Proteins / Peptides
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Thermal Denaturation of Proteins Studied by UV Spectroscopy Natasa Poklar and Gorazd Vesnaver
UV spectroscopy has been widely used for monitoring the unfolding of proteins. During temperature-induced denaturation the protein absorbance changes with temperature until the process of unfolding is completed. A determination of DH(Td) and DS(Td) for a-chymotrypsinogen A at pH 3.0 is demonstrated.
Poklar, Natasa; Vesnaver, Gorazd. J. Chem. Educ. 2000, 77, 380.
Biophysical Chemistry |
UV-Vis Spectroscopy |
Undergraduate Research |
Proteins / Peptides |
Molecular Properties / Structure
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Chromatin Isolation and DNA Sequence Analysis in Large Undergraduate Laboratory Sections Ann E. Hagerman
One exercise is a simple laboratory experiment in which chromatin is isolated from chicken liver and is resolved into histone proteins and DNA by ion-exchange chromatography. The other is a series of computer simulations that introduce DNA sequencing, mapping, and sequence analysis to the students.
Hagerman, Ann E. J. Chem. Educ. 1999, 76, 1426.
Biotechnology |
Proteins / Peptides |
Nucleic Acids / DNA / RNA
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Homogeneous Immunoassays: Historical Perspective and Future Promise Edwin F. Ullman
The founding and growth of Syva Company is examined in the context of its leadership role in the development of homogeneous immunoassays. The simple mix and read protocols of these methods offer advantages in routine analytical and clinical applications.
Ullman, Edwin F. J. Chem. Educ. 1999, 76, 781.
Biotechnology |
Enzymes |
Atomic Properties / Structure |
Free Radicals |
Photochemistry |
Medicinal Chemistry |
Proteins / Peptides
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Immunoassay, DNA Analysis, and Other Ligand Binding Assay Techniques: From Electropherograms to Multiplexed, Ultrasensitive Microarrays on a Chip Roger P. Ekins
"Ligand" or "binding" assays have made a major impact on biomedical research and clinical diagnosis since their development in the late 1950s. Immunoassay techniques (relying on specific antibodies to bind the target analyte) represent the best-known example, but analogous DNA and RNA analysis methods (using oligonucleotides to recognize defined polynucleotide sequences) are rapidly gaining in importance and are likely to exert profound effects on human society.
Ekins, Roger P. J. Chem. Educ. 1999, 76, 769.
Hormones |
Instrumental Methods |
Molecular Recognition |
Nanotechnology |
Proteins / Peptides |
Biotechnology
|
Molecular Mechanics across Chemistry (by Anthony K. Rapp and Carla J. Casewit) G. Craig Hill
The text is divided into 8 chapters, beginning with an Overview, followed by Organics, Peptides and Proteins, Drug Design, DNA, Synthetic Polymers, Inorganics, and Force Fields.
Hill, G. Craig. J. Chem. Educ. 1999, 76, 325.
Proteins / Peptides |
Drugs / Pharmaceuticals
|
Showing Food Foams Properties with Common Dairy Foods Carlos Bravo-Diaz and Elisa Gonzalez-Romero
Some easy to carry, inexpensive and safe experiments developed using familiar kitchen materials related with egg foams. Eggs's properties are not only limited to prepare excellent and delicious emulsions like mayonnaise but also makes excellent foams, increasing their volume significantly in two primary ways.
Bravo-Diaz, Carlos; Gonzalez-Romero, Elisa. J. Chem. Educ. 1997, 74, 1133.
Learning Theories |
Food Science |
Proteins / Peptides |
Applications of Chemistry
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The Sweetness of Aspartame: A Biochemistry Lab for Health Science Chemistry Courses Paul J. Stein
A laboratory exercise to study the effect of aspartame concentration on sweetness has been developed. Class data is averaged and plotted on the same graph as absorbance and taste rating vs. [aspartame]. The absorbance plot follows Beer's law while the taste rating plot displays the hyperbolic response of protein-ligand binding plots.
Stein, Paul J. J. Chem. Educ. 1997, 74, 1112.
Food Science |
Nonmajor Courses |
Proteins / Peptides |
Medicinal Chemistry
|
Microburger Biochemistry: Extraction and Spectral Characterization of Myoglyobin from Hamburger Sheri A. Bylkas and Laura A. Andersson
The protocol combines protein extraction, oxidation and reduction, and simple spectroscopic analysis, as well as gel filtration chromatography and generation/analysis of spectral scans. Mammalian myoglobin (Mb) is easily extracted from steak, to illustrate and address why fresh meat is red and aged meat is brown; the protein has unique spectral properties that are diagnostic for characterization of sample identity.
Bylkas, Sheri A.; Andersson, Laura A. J. Chem. Educ. 1997, 74, 426.
Laboratory Equipment / Apparatus |
Proteins / Peptides |
Oxidation / Reduction |
UV-Vis Spectroscopy |
Chromatography
|
The Use of a "Qual" Centrifuge for Greatly Simplifying and Speeding the Study of Milk Quentin R. Petersen
Laboratory study of the constituents of milk is almost always slowed by difficult separation of relatively large amounts of curd and whey by filtration. In the two-and-one-half hour experiment described, only 5 mL of skim milk is used and the curd is separated from the whey by using a simple "qual" centrifuge.
J. Chem. Educ. 1996, 73, 848.
Proteins / Peptides |
Carbohydrates |
Qualitative Analysis
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In Focus. Protein Structure (Darb, N. J.; Creighton, T. E.; Rickwood, David; Male, David)
Continuing series.
J. Chem. Educ. 1995, 72, A94.
Proteins / Peptides |
Molecular Properties / Structure
|
A Ligand-Binding Study Based on a Protein Assay Method Chial, H. J.; Congdon, R. W.; Splittgerber, A. G.
Experimental procedure for determining the protein concentration of an unknown solution, as well as the number of dye-binding sites on the protein; data and analysis included.
Chial, H. J.; Congdon, R. W.; Splittgerber, A. G. J. Chem. Educ. 1995, 72, 76.
Proteins / Peptides |
Coordination Compounds |
Crystal Field / Ligand Field Theory
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Determination of Ligand- Macromolcule Binding Constants by a Competative Spectrofluorometric Technique: Caffeine Binding to Human Serum Albumin Inestal, J. J.; Gonzalez-Velasco, F.; Ceballos, A.
Through the use of a relatively simple procedure this laboratory experiment will give the student experience in an important subject in biomedical sciences: the binding of small molecules to proteins.
Inestal, J. J.; Gonzalez-Velasco, F.; Ceballos, A. J. Chem. Educ. 1994, 71, A297.
Fluorescence Spectroscopy |
Crystal Field / Ligand Field Theory |
Proteins / Peptides |
Medicinal Chemistry
|
A Simple Competitive Protein-Binding Experiment Anderson, Alexander J.
This experiment was developed for teaching the concept of competitive protein-binding assays, but with the use of inexpensive reagents and commonly available laboratory equipment in place of specialized immunoassay reagents and radiation counting equipment.
Anderson, Alexander J. J. Chem. Educ. 1994, 71, 994.
Crystal Field / Ligand Field Theory |
Proteins / Peptides
|
Use of Tangle Links To Show Globular Protein Structure Marino, Francis
Using Tangle Links (a child's toy) to model the primary, secondary, tertiary, and quaternary structure of proteins.
Marino, Francis J. Chem. Educ. 1994, 71, 741.
Proteins / Peptides |
Molecular Modeling
|
Gelatin-A Compound for All Reasons Pal, Tarasankar
Gelatin may produce effective reagents for a wide variety of applications in chemistry, including pharmaceutical, environmental, and biomedical applications.
Pal, Tarasankar J. Chem. Educ. 1994, 71, 679.
Proteins / Peptides |
Drugs / Pharmaceuticals |
Medicinal Chemistry
|
A family of hydrogen bonds in the model system salicylic acid-toluene-water: A biophysical experiment modeled on the distribution of the monomeric acid between two phases and on its dimerization in the organic phase Worley, John D.
Hydrogen bonding is often studied using model systems: the system salicylic acid-tolune-water offers an interesting example for study by students in a physical or biophysical chemistry lab.
Worley, John D. J. Chem. Educ. 1993, 70, 417.
Hydrogen Bonding |
Proteins / Peptides |
Water / Water Chemistry |
Equilibrium
|
An inexpensive gel-filtration chromatography experiment: A simple biochemical laboratory exercise for high school and undergraduate students who make their own column and apply simple detection techniques Rowe, H. Alan
The experiment described in this paper can be conducted with a minimum of supplies and equipment at the high school or undergraduate level thus lowering the usual barriers an exercise of this type presents.
Rowe, H. Alan J. Chem. Educ. 1993, 70, 415.
Chromatography |
Bioorganic Chemistry |
Laboratory Equipment / Apparatus |
Carbohydrates |
Proteins / Peptides
|
Teaching bioorganic chemistry: An introductory course Dugas, Hermann
Bioorganic chemistry could be defined as a discipline that is essentially concerned with using the tools of organic chemistry to understand biochemical processes.
Dugas, Hermann J. Chem. Educ. 1992, 69, 268.
Bioorganic Chemistry |
Catalysis |
Biological Cells |
Proteins / Peptides |
Medicinal Chemistry
|
Methods for obtaining the midpoint potential E(m,pH) of a bacterial chromophore. Rodriguez, Frdric; Prats, Michel.
The authors present a critical analysis of three methods that are used to determine the carotenoid midpoint potential of chromatophores extracted from Rhodobacter capsulatus.
Rodriguez, Frdric; Prats, Michel. J. Chem. Educ. 1992, 69, 245.
Proteins / Peptides |
Photochemistry |
Biological Cells |
Bioorganic Chemistry
|
A less hazardous chromic acid substitute for cleaning glassware Manske, Paul M.; Stimpfel, Teresa M.; Gershey, Edward L.
Evaluation of several common cleaning solutions for their ability to remove known amounts of baked-on lipid or protein from glassware.
Manske, Paul M.; Stimpfel, Teresa M.; Gershey, Edward L. J. Chem. Educ. 1990, 67, A280.
Laboratory Management |
Lipids |
Proteins / Peptides
|
Teaching biotechnology Brooks, Helen B.; Brooks, David W.; Schuster, Sheldon M.; Wylie, Dwane E.
121. Bits and pieces, 45. HyperCard stack of the commonly used nucleic acid and protein techniques of biotechnology.
Brooks, Helen B.; Brooks, David W.; Schuster, Sheldon M.; Wylie, Dwane E. J. Chem. Educ. 1990, 67, 1033.
Biotechnology |
Proteins / Peptides
|
Effects of "crowding" in protein solutions Ralston, G. B.
The effects of macromolecular nonideality and crowding on chemical equilibria, association reactions, and enzyme kinetics.
Ralston, G. B. J. Chem. Educ. 1990, 67, 857.
Proteins / Peptides |
Equilibrium |
Reactions |
Enzymes |
Kinetics
|
Selective ion transport across biological membranes: A simple experiment in bioinorganic chemistry Adams, Paul A.; Black, Duncan
In this demonstration the intact human erythrocyte is utilized as a simple model with which to demonstrate the essential features of facilitated anion transport across biological membranes.
Adams, Paul A.; Black, Duncan J. Chem. Educ. 1990, 67, 526.
Bioinorganic Chemistry |
Proteins / Peptides |
Ion Exchange
|
Ferrimyoglobin-fluoride: An undergraduate kinetics experiment Russo, Steven O.; Hanania, George I. H.
The authors propose an experiment which concerns the reaction of the protein ferrimyoglobin with fluoride ion, in dilute aqueous solution.
Russo, Steven O.; Hanania, George I. H. J. Chem. Educ. 1990, 67, 352.
Kinetics |
Proteins / Peptides |
Aqueous Solution Chemistry
|
Isolation of the active site of cytochrome c by reverse-phase high-performance liquid chromatography Kenigsberg, Paul A.; Blanke, Steven R.; Hager, Lowell P.
To serve as an introduction to research, an advanced-level graduate laboratory course should be challenging, incorporate currently relevant techniques, offer more responsibility to students, and contain a minimum of technical pitfalls. The following experiment meets all the aforementioned criteria.
Kenigsberg, Paul A.; Blanke, Steven R.; Hager, Lowell P. J. Chem. Educ. 1990, 67, 177.
HPLC |
Proteins / Peptides
|
An enzyme immunoassay for human transferrin Russo, Salvatore F.; Dahlberg, Judy Utter
The field of immunology provides a powerful method for detecting specific noncatalytic proteins.
Russo, Salvatore F.; Dahlberg, Judy Utter J. Chem. Educ. 1990, 67, 175.
Enzymes |
Medicinal Chemistry |
Proteins / Peptides
|
Azosulfonamides: Preparation and binding to carbonic anhydrase: A bioorganic chemistry experiment Manalang, Mary G.; Bundy, Hallie F.
Preparation of azosulfonamide, visible absorption analysis of free and enzyme-bond azosulfonamide, and titration of BCA with azosulfonamide.
Manalang, Mary G.; Bundy, Hallie F. J. Chem. Educ. 1989, 66, 609.
Bioorganic Chemistry |
Synthesis |
Proteins / Peptides |
Titration / Volumetric Analysis |
Enzymes
|
Lecture demonstrations for organic/ biochemistry allied health courses Deavor, James P.
Simple demonstrations on enantiomeric pairs and protein structure.
Deavor, James P. J. Chem. Educ. 1988, 65, 622.
Enantiomers |
Chirality / Optical Activity |
Proteins / Peptides |
Nonmajor Courses |
Amino Acids
|
A convenient synthesis of aspartame Lindeberg, Gunnar
The one tube, enzymatic synthesis of the dipeptide sweetener aspartame.
Lindeberg, Gunnar J. Chem. Educ. 1987, 64, 1062.
Synthesis |
Enzymes |
Proteins / Peptides
|
Protein structure prediction in color Davis, Lawrence C.; Radke, Gary A.
84. Implementing the Chuo and Faslar method for prediction of secondary structure and the Kyte and Doolittle method for predicting "hydropathic character" on an Apple IIe computer with color graphics.
Davis, Lawrence C.; Radke, Gary A. J. Chem. Educ. 1987, 64, 582.
Proteins / Peptides |
Molecular Properties / Structure
|
An inexpensive molecular model Brickley, Meredith; Silva, R. A.
An inexpensive, space-filling model to help students understand the structure of proteins.
Brickley, Meredith; Silva, R. A. J. Chem. Educ. 1985, 62, 1077.
Molecular Modeling |
Proteins / Peptides
|
Use of the "cubic snake" as a molecular model Gilon, Chaim
Using a "cubic snake" to model macromolecules.
Gilon, Chaim J. Chem. Educ. 1985, 62, 1074.
Molecular Modeling |
Molecular Properties / Structure |
Proteins / Peptides |
Enzymes
|
The catalytic function of enzymes Splittgerber, Allan G.
Review of the structure, function, and factors that influence the action of enzymes.
Splittgerber, Allan G. J. Chem. Educ. 1985, 62, 1008.
Catalysis |
Enzymes |
Mechanisms of Reactions |
Proteins / Peptides |
Molecular Properties / Structure
|
Zinc enzymes Bertini, I.; Luchinat, C.; Monnanni, R.
The role played by catalytic and noncatalytic zinc in biochemical systems. From the "State of the Art Symposium: Bioinorganic Chemistry", held at the ACS meeting, Miami, 1985.
Bertini, I.; Luchinat, C.; Monnanni, R. J. Chem. Educ. 1985, 62, 924.
Enzymes |
Bioinorganic Chemistry |
Lewis Acids / Bases |
Proteins / Peptides
|
Bomb calorimetry: The energy content of pizza Stout, Roland P.; Nettleton, Faith E.; Price, Lynn M.
Determining the energy content and percent protein of pizza using bomb calorimetry.
Stout, Roland P.; Nettleton, Faith E.; Price, Lynn M. J. Chem. Educ. 1985, 62, 438.
Calorimetry / Thermochemistry |
Food Science |
Proteins / Peptides |
Titration / Volumetric Analysis
|
Methemoglobinemia: An illness caused by the ferric state Senozan, N. M.
Hemoglobin's ability to carry oxygen depends on the iron being in the +2 state; methemoglobinemia involves the oxidation of hemoglobin iron to the +3 state.
Senozan, N. M. J. Chem. Educ. 1985, 62, 181.
Proteins / Peptides |
Enzymes |
Medicinal Chemistry |
Oxidation / Reduction |
Oxidation State
|
Gel filtration chromatography: A laboratory experiment Hurlbut, Jeffrey A.; Schonbeck, Niels D.
A rapid, visual demonstration of protein separation by gel filtration chromatography.
Hurlbut, Jeffrey A.; Schonbeck, Niels D. J. Chem. Educ. 1984, 61, 1021.
Chromatography |
Separation Science |
Proteins / Peptides
|
Perspectives in biochemistry: Methods for DNA sequencing Wood, Anne T.
Describes how DNA is sequenced.
Wood, Anne T. J. Chem. Educ. 1984, 61, 886.
Proteins / Peptides |
Biotechnology |
Electrophoresis
|
The synthesis of a dipeptide from its component amino acids: Protecting groups in the elementary organic laboratory Young, Paul E.; Campbell, Andrew
A three-step procedure for synthesizing a dipeptide from its component amino acids.
Young, Paul E.; Campbell, Andrew J. Chem. Educ. 1982, 59, 701.
Synthesis |
Amino Acids |
Proteins / Peptides
|
Protein denaturation: A physical chemistry project lab Pickering, Miles; Crabtree, Robert H.
This experiment links physical chemistry with biology and can be done with in advanced freshman course.
Pickering, Miles; Crabtree, Robert H. J. Chem. Educ. 1981, 58, 513.
Proteins / Peptides |
Biophysical Chemistry |
Molecular Properties / Structure
|
Fractionation of gels following electrophoresis Racusen, David
A simple and inexpensive technique for slicing electrophoresis gels.
Racusen, David J. Chem. Educ. 1979, 56, 628.
Separation Science |
Electrophoresis |
Proteins / Peptides |
Laboratory Management |
Laboratory Equipment / Apparatus |
Biotechnology
|
Isoenzymes Daugherty, N. A.
The separation, identification, and measurement of isoenzymes is an appropriate topic for a special lecture in general chemistry.
Daugherty, N. A. J. Chem. Educ. 1979, 56, 442.
Enzymes |
Proteins / Peptides |
pH |
Electrophoresis |
Separation Science |
Electrochemistry |
Applications of Chemistry
|
Compact Compacts Huebner, Jay S.; Shiflett, R. B.; Blanck, Harvey F.
A collection of three suggestions regarding demonstrating the oxidation of hydrocarbons and the primary, secondary, and tertiary structure of proteins and the first law of thermodynamics as applied to air conditioning.
Huebner, Jay S.; Shiflett, R. B.; Blanck, Harvey F. J. Chem. Educ. 1979, 56, 389.
Oxidation / Reduction |
Alkanes / Cycloalkanes |
Molecular Properties / Structure |
Proteins / Peptides |
Thermodynamics
|
The separation of chymotrypsin and chymotrypsinogen: An affinity chromatography experiment for biological chemistry students Branchini, Bruce; Ziolkowski, Rodney
An experiment that can be used as either a demonstration or a laboratory illustrating the principles of affinity chromatography to purify an enzyme.
Branchini, Bruce; Ziolkowski, Rodney J. Chem. Educ. 1979, 56, 281.
Separation Science |
Chromatography |
Proteins / Peptides
|
Compact compact Nelson, Gregory V.
Using a cardboard mailing tube to demonstrate the rigidity of the folding pattern and the flexibility of a non-helical region in a protein.
Nelson, Gregory V. J. Chem. Educ. 1977, 54, 578.
Molecular Modeling |
Molecular Properties / Structure |
Proteins / Peptides
|
Molecular membrane model Huebner, J. S.
Making a model of a lipid bilayer using polystyrene balls and pipe cleaners.
Huebner, J. S. J. Chem. Educ. 1977, 54, 171.
Membranes |
Lipids |
Molecular Modeling |
Proteins / Peptides |
Dyes / Pigments
|
Amino acid sequence diversity in proteins Blackman, David
The number of unique proteins that can be generated from a small number of amino acids is truly enormous.
Blackman, David J. Chem. Educ. 1977, 54, 170.
Proteins / Peptides |
Amino Acids
|
Bromolain. Experiments illustrating proteolytic enzyme action Reigh, Darryel L.
The following set of exercises provides a simple visual method of demonstrations some of the characteristics of enzymes in general, such as heat and pH lability and inhibition, as well as some specific properties of bromelain intide hydrolysis. These experiments can be used with freshman in introductory courses and juniors in biochemistry.
Reigh, Darryel L. J. Chem. Educ. 1976, 53, 386.
Enzymes |
pH |
Food Science |
Natural Products |
Proteins / Peptides
|
Non-covalent interactions: Key to biological flexibility and specificity Frieden, Earl
Summarizes the types of non-covalent interactions found among biomolecules and how they facilitate the function of antibodies, hormones, and hemoglobin.
Frieden, Earl J. Chem. Educ. 1975, 52, 754.
Noncovalent Interactions |
Hydrogen Bonding |
Water / Water Chemistry |
Proteins / Peptides |
Amino Acids |
Molecular Properties / Structure |
Hormones
|
Allied health chemistry laboratory. Amino acids, insulin, proteins, and skin Dever, David F.
Describes an allied health chemistry laboratory involving amino acids, insulin, proteins and skin that begins with the construction of molecular models of amino acids and proteins.
Dever, David F. J. Chem. Educ. 1975, 52, 338.
Amino Acids |
Proteins / Peptides |
Medicinal Chemistry |
Nonmajor Courses |
Molecular Properties / Structure |
Molecular Modeling |
Applications of Chemistry
|
Hemoglobin. Isolation and chemical properties Russo, Salvatore F.; Sorstokke, Ralph B.
Summarizes the properties of hemoglobin and demonstrates the colors of oxyhemoglobin, deoxyhemoglobin, methemoglobin, nitrosylhemoglobin; examines their visible absorption spectra; follows the rate of reaction of oxyhemoglobin with nitrite spectrophotometrically; and the effect of salt concentration on the integrity of red blood cells.
Russo, Salvatore F.; Sorstokke, Ralph B. J. Chem. Educ. 1973, 50, 347.
Proteins / Peptides
|
Sulfur compounds. Pollution, health effects, and biological function Leh, F.; Chan, K. M.
Reviews the role of sulfur and its compounds in pollution and adverse health effects as well as biological functions.
Leh, F.; Chan, K. M. J. Chem. Educ. 1973, 50, 246.
Nonmetals |
Proteins / Peptides
|
Photochemical reactions of natural macromolecules. Photoreactions of proteins Neckers, Douglas C.
Reviews salient features of protein destruction by ultraviolet radiation.
Neckers, Douglas C. J. Chem. Educ. 1973, 50, 164.
Photochemistry |
Proteins / Peptides |
Amino Acids
|
Questions [and] Answers Campbell, J. A.
Five questions requiring an application of chemical principles and their solutions.
Campbell, J. A. J. Chem. Educ. 1973, 50, 128.
Enrichment / Review Materials |
Proteins / Peptides |
pH |
Carbohydrates |
Molecular Properties / Structure
|
A unified apparatus for paper and gel electrophoresis Racusen, David; White, Lee
Presents an apparatus that can be used for either paper and gel electrophoresis.
Racusen, David; White, Lee J. Chem. Educ. 1972, 49, 439.
Electrophoresis |
Separation Science |
Amino Acids |
Proteins / Peptides
|
Questions [and] Answers Campbell, J. A.
Five questions requiring an application of basic chemical principles.
Campbell, J. A. J. Chem. Educ. 1972, 49, 269.
Enrichment / Review Materials |
Agricultural Chemistry |
Metabolism |
Calorimetry / Thermochemistry |
Proteins / Peptides
|
Sickle-cell anemia, hemoglobin solubility and resistance to malaria Martin, David L.; Huheey, James E.
The phenomenon of erythrocytic sickling is of considerable pedagogical usefulness since it provides a bridge of relevance between chemistry and biology.
Martin, David L.; Huheey, James E. J. Chem. Educ. 1972, 49, 177.
Proteins / Peptides |
Medicinal Chemistry |
Applications of Chemistry
|
Preparation of the proteins by micro-organisms Dieteren, H. M. L.; Schouteten, A. P. H.
Yeast are used to generate proteins from tetradecane in a controlled vessel.
Dieteren, H. M. L.; Schouteten, A. P. H. J. Chem. Educ. 1970, 47, 663.
Proteins / Peptides |
Synthesis
|
Hydrogen sulfide under any other name still smells. A poisonous story Brasted, Robert C.
The chemistry of hydrogen sulfide affords an excellent opportunity to integrate descriptive inorganic and coordination chemistry with biochemistry.
Brasted, Robert C. J. Chem. Educ. 1970, 47, 574.
Descriptive Chemistry |
Molecular Properties / Structure |
Coordination Compounds |
Enzymes |
Proteins / Peptides
|
Models for tertiary structures: Myoglobin and lysozyme Smith, Ivor; Smith, Margaret J.; Roberts, Lynne
Presents the design details for constructing three dimensional models of proteins, including myoglobin and lysozyme.
Smith, Ivor; Smith, Margaret J.; Roberts, Lynne J. Chem. Educ. 1970, 47, 302.
Molecular Properties / Structure |
Molecular Modeling |
Proteins / Peptides |
Hydrogen Bonding |
Noncovalent Interactions
|
Disposable macromolecular model "kits" Nicholson, Isadore
A brief note suggesting the use of colored pipe cleaners for the construction of three dimensional models of polymers, particularly enzymes and other proteins.
Nicholson, Isadore J. Chem. Educ. 1969, 46, 671.
Molecular Modeling |
Enzymes |
Proteins / Peptides
|
Disc electrophoresis Brewer, John M.; Ashworth, Raymond B.
Describes the procedures involved in separating proteins or nucleic acids through disc electrophoresis.
Brewer, John M.; Ashworth, Raymond B. J. Chem. Educ. 1969, 46, 41.
Electrochemistry |
Electrophoresis |
Proteins / Peptides |
Separation Science
|
Simple construction to determine protein molecular weights by the osmotic pressure method Candlish, John K.
This short note presents a simple device to determine protein molecular weights through osmotic pressure.
Candlish, John K. J. Chem. Educ. 1968, 45, 93.
Molecular Properties / Structure |
Proteins / Peptides |
Physical Properties
|
Errors in representing structures of proteins and nucleic acids Day, Richard A.; Ritter, Edmond J.
It is the exceptional modern textbook in biochemistry or organic chemistry that consistently represents the structures of proteins and nucleic acids as they are found to exist in nature.
Day, Richard A.; Ritter, Edmond J. J. Chem. Educ. 1967, 44, 761.
Proteins / Peptides |
Molecular Properties / Structure
|
Polyethylene and pipecleaner models of biological polymers Pollard, Harvey Bruce
An accurate method for modeling polysaccharides, nucleic acids, and proteins involves the use of pipecleaners, polyethylene tubing, and proteins.
Pollard, Harvey Bruce J. Chem. Educ. 1966, 43, 327.
Proteins / Peptides |
Molecular Modeling |
Molecular Properties / Structure |
Carbohydrates
|
Ribonucleic acid: The simplest information-transmitting molecule Fraenkel-Conrat, H.
Examines the chemical structure of nucleic acids, chemical modification of RNA and mutagenesis, role of RNA in protein structure, the mechanism of information transfer from RNA to protein, and mutants and coding.
Fraenkel-Conrat, H. J. Chem. Educ. 1963, 40, 216.
Proteins / Peptides
|
Protein nitrogen in fruits, vegetables, and meats by a modified Kjeldahl method: A quantitative analysis experiment Meloan, Clifton E.
This "research" type experiment involves the quantitative determination of nitrogen in such foods as fruits, vegetables, and meats.
Meloan, Clifton E. J. Chem. Educ. 1962, 39, 365.
Quantitative Analysis |
Proteins / Peptides
|
Identification of amino acids in a protein hydrolysate by paper chromatography Clapp, L. B.; Hansch, Corwin
By varying the number of amino acids to be identified, the difficulty of this procedure can be easily controlled.
Clapp, L. B.; Hansch, Corwin J. Chem. Educ. 1960, 37, 293.
Amino Acids |
Qualitative Analysis |
Proteins / Peptides |
Chromatography
|
The geometry of giant molecules Price, Charles C.
The author examines a variety of specific examples of natural and synthetic polymer molecules and describes how their geometric molecular arrangements influence their properties.
Price, Charles C. J. Chem. Educ. 1959, 36, 160.
Molecular Properties / Structure |
Proteins / Peptides |
Carbohydrates
|
Schematic models of biochemical polymers Blackwell, R. Quentin
Demonstrates the use of plastic necklace beads to represent polysaccharides, peptides and proteins, and nucleotides.
Blackwell, R. Quentin J. Chem. Educ. 1957, 34, 500.
Molecular Modeling |
Molecular Properties / Structure |
Proteins / Peptides |
Carbohydrates
|
Potentialities of protein isomerism Asimov, Isaac
The permutations generated by structural isomerism in proteins could be demonstrated more convincingly and realistically if the amino acid compositions of actual proteins were taken into consideration.
Asimov, Isaac J. Chem. Educ. 1954, 31, 125.
Proteins / Peptides |
Molecular Properties / Structure |
Amino Acids |
Constitutional Isomers
|
Osmotic pressure and molecular weight Hitchcock, David I.
Discusses the use of osmotic pressure to establish the molecular weights of proteins and synthetic polymers.
Hitchcock, David I. J. Chem. Educ. 1951, 28, 478.
Molecular Properties / Structure |
Chemometrics |
Aqueous Solution Chemistry |
Proteins / Peptides
|
Teaching amino acid formulation Patton, A. R.
Presents a device that aids in explaining the concept of dipolar ions necessary to an understanding of the behavior of amino acids and proteins.
Patton, A. R. J. Chem. Educ. 1951, 28, 471.
Amino Acids |
Proteins / Peptides |
Acids / Bases |
pH
|
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