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If You Were a Molecule in a Chromatography Column, What Would You See?John Mattice To visualize what takes place in a chromatography column, enlarge the molecules to human size and expand the columns to keep the ratio of size of molecule to size of column the same. Using such a model, this article explains how band broadening occurs as a result of resistance to mass transfer, multiple flow paths, and stagnant mobile phase. Mattice, John. J. Chem. Educ.2008, 85, 925.
Integrating Advanced High School Chemistry Research with Organic Chemistry and Instrumental Methods of AnalysisBrian J. Kennedy Describes the unique opportunities in chemistry available at a science and technology magnet high school. Students may select entry-level courses such as honors and advanced placement chemistry, take electives in organic chemistry with instrumental methods of analysis, and ultimately complete a senior chemical analysis research course. Kennedy, Brian J. J. Chem. Educ.2008, 85, 393.
ChromatographyEd Vitz, John W. Moore A section of ChemPrime, the Chemical Educations Digital Library's free General Chemistry textbook.
Chromatography |
Gas Chromatography
Chromatography, Paper (ChemPages Lab)John W. Moore, Jerrold J. Jacobsen, Joe L. March Chromatography, Paper: this is a resource in the collection "ChemPages Laboratory Resources". Paper chromatography is one method for testing the purity of compounds and identifying substances. Paper chromatography is a useful technique because it is relatively quick and requires small quantities of material. The ChemPages Laboratory Resources are a set of web pages that include text, images, video, and self check questions. The topics included are those that are commonly encountered in the first-year chemistry laboratory. They have been put together for use as both a pre-laboratory preparation tool and an in-laboratory reference source.
Chromatography
Principles of Gel Permeation ChromatographyGuilherme Andrade Marson, Bayardo Baptista Torres Principles of Gel Permeation Chromatography presents the principles of gel permeation chromatography (GPC) for students in introductory undergraduate courses of chemistry and biochemistry. These principles are presented in four sections: Introduction, Real Lab, Virtual Lab, and Microscopic Model. The Introduction and Real Lab sections present a brief view of the basic experimental apparatus typically used in laboratory GPC in order to provide a concrete connection of the real process of separation. The basic elements of column chromatography, emphasizing the stationary and mobile phases, are presented in the Introduction, followed by a sequence of pictures and texts describing major steps in GPC analysis in the Real Lab section. The Virtual Lab section is a simulator. Three samples are available for a virtual GPC experiment: sample 1, containing hemoglobin; sample 2, containing methylene blue; and sample 3, containing both methylene blue and hemoglobin. Each sample undergoes a virtual separation run, which is dynamically represented in three ways in the software: a virtual column, the collected fractions, and a virtual chromatogram. This threefold representation allows the simultaneous view of key aspects of the process to demonstrate the correlation between the experimental procedure and the resulting chromatogram.
